Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | A0A140N5X9 | - |
- |
Purification (Comment) | Organism |
---|---|
a chimera protein of RrmJ fused to a chitin-binding domain was purified by affinity chromatography on a chitin affinity column | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + uridine2552 in 23S rRNA | identification of the methylated nucleotide as 2'-O-methyluridine 2552, by reverse phase high performance liquid chromatography analysis, boronate affinity chromatography, and hybridization-protection experiments. In vitro, FtsJ does not efficiently methylate ribosomes purified from a strain producing FtsJ, suggesting that these ribosomes are already methylated in vivo by FtsJ. FtsJ is active on ribosomes and on the 50 S ribosomal subunit, but is inactive on free rRNA, suggesting that its natural substrate is ribosomes or a pre-ribosomal ribonucleoprotein particle | Escherichia coli | S-adenosyl-L-homocysteine + 2'-O-methyluridine2552 in 23S rRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
23 S ribosomal RNA methyltransferase | - |
Escherichia coli |
FTSJ | - |
Escherichia coli |
FtsJ/RrmJ heat shock protein | - |
Escherichia coli |
RrmJ | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Escherichia coli |