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Literature summary for 2.1.1.165 extracted from
- cDNA cloning of Batis maritima methyl chloride transferase and purification of the enzyme (1998), Proc. Natl. Acad. Sci. USA, 95, 12866-12871.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Batis maritima |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | the disparity between the observed (22500 Da) and calculated molecular mass (25761 Da) suggests that the methylase undergoes posttranslational cleavage, possibly during purification | Batis maritima |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22474 | - |
x * 22474, the methylase can function in either a monomeric or oligomeric form, mass spectrometry | Batis maritima |
| 22500 | - |
x * 22500, the methylase can function in either a monomeric or oligomeric form, SDS-PAGE | Batis maritima |
| 25761 | - |
x * 25761, the methylase can function in either a monomeric or oligomeric form, calculated from sequence | Batis maritima |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + chloride | Batis maritima | an obvious function for a halophytic methylase would be the maintenance of homeostatic levels of cytoplasmic chloride ion. The secretion of excess chloride into the soil could not greatly benefit a halophytic plant. On the other hand, the synthesis and distillation of a volatile gas, methyl chloride, into the atmosphere could be a useful mechanism for disposing of excess chloride | S-adenosyl-L-homocysteine + methyl chloride | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Batis maritima | Q9ZSZ7 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 2700fold to homogeneity by ammonium sulfate fractionation, gel filtration, adenosine affinity chromatography, and a second step of gel filtration | Batis maritima |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Batis maritima | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.3 | - |
purified enzyme, substrate iodide | Batis maritima |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, enzyme forms an aggregate with molecular mass of approximately 500000 Da | Batis maritima |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | purified enzyme is unable to use bisulfide (HS-) as an acceptor | Batis maritima | ? | - |
? | |
| S-adenosyl-L-methionine + chloride | - |
Batis maritima | S-adenosyl-L-homocysteine + methyl chloride | - |
? | |
| S-adenosyl-L-methionine + chloride | an obvious function for a halophytic methylase would be the maintenance of homeostatic levels of cytoplasmic chloride ion. The secretion of excess chloride into the soil could not greatly benefit a halophytic plant. On the other hand, the synthesis and distillation of a volatile gas, methyl chloride, into the atmosphere could be a useful mechanism for disposing of excess chloride | Batis maritima | S-adenosyl-L-homocysteine + methyl chloride | - |
? | |
| S-adenosyl-L-methionine + iodide | iodide is the preferred substrate | Batis maritima | S-adenosyl-L-homocysteine + methyl iodide | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 22474, the methylase can function in either a monomeric or oligomeric form, mass spectrometry | Batis maritima |
| ? | x * 22500, the methylase can function in either a monomeric or oligomeric form, SDS-PAGE | Batis maritima |
| ? | x * 25761, the methylase can function in either a monomeric or oligomeric form, calculated from sequence | Batis maritima |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MCT | - |
Batis maritima |
| methyl chloride transferase | - |
Batis maritima |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at room temperature | Batis maritima |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
assay at | Batis maritima |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| S-adenosyl-L-methionine | - |
Batis maritima | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Batis maritima | calculated from sequence | - |
5.1 |
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