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Literature summary for 2.1.1.163 extracted from

  • Wissenbach, U.; Ternes, D.; Unden, G.
    An Escherichia coli mutant containing only demethylmenaquinone, but not menaquinone: effects on fumarate, dimethylsulfoxide, trimethylamine N-oxide and nitrate respiration (1992), Arch. Microbiol., 158, 68-73.
    View publication on PubMed
Search for more literature for 2.1.1.163

Protein Variants

Protein Variants Comment Organism
additional information mutant ubiE lacks ubiquinone due to defect in a specific methylation step of ubiquinone synthesis. Synthesis of menaquinone from demethylmenaquinone depends on the same gene ubiE. Mutant contains only demethylmenaquinone, but not menaquinone. Strain is able to grow with fumarate, trimethylamine N-oxide and dimethylsulfoxide, but not with nitrate as electron acceptor. Anaerobic respiration with fumarate and trimethylamine are catalyzed at 69% and 74% of wild-type rates, respectively. Dimethylsulfoxide respiration is reduced to 38% of wild-type, and nitrate respiration is below 8% Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-octaprenyl-1,4-naphthoquinone + S-adenosyl-L-methionine Escherichia coli
-
 2-octaprenyl-3-methyl-1,4-naphthoquinone + S-adenosyl-L-homocysteine i.e. menaquinone ?
2-octaprenyl-1,4-naphthoquinone + S-adenosyl-L-methionine Escherichia coli AN387
-
 2-octaprenyl-3-methyl-1,4-naphthoquinone + S-adenosyl-L-homocysteine i.e. menaquinone ?
2-octaprenyl-6-methoxy-1,4-benzoquinone + S-adenosyl-L-methionine Escherichia coli
-
 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinone + S-adenosyl-L-homocysteine
-
 ?
2-octaprenyl-6-methoxy-1,4-benzoquinone + S-adenosyl-L-methionine Escherichia coli AN387
-
 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinone + S-adenosyl-L-homocysteine
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli
-
 gene ubiE
-
Escherichia coli AN387
-
 gene ubiE
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-octaprenyl-1,4-naphthoquinone + S-adenosyl-L-methionine
-
 Escherichia coli 2-octaprenyl-3-methyl-1,4-naphthoquinone + S-adenosyl-L-homocysteine i.e. menaquinone ?
2-octaprenyl-1,4-naphthoquinone + S-adenosyl-L-methionine
-
 Escherichia coli AN387 2-octaprenyl-3-methyl-1,4-naphthoquinone + S-adenosyl-L-homocysteine i.e. menaquinone ?
2-octaprenyl-6-methoxy-1,4-benzoquinone + S-adenosyl-L-methionine
-
 Escherichia coli 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinone + S-adenosyl-L-homocysteine
-
 ?
2-octaprenyl-6-methoxy-1,4-benzoquinone + S-adenosyl-L-methionine
-
 Escherichia coli AN387 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinone + S-adenosyl-L-homocysteine
-
 ?

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Escherichia coli

General Information

General Information Comment Organism
malfunction in the uniE-lacking mutant strain AN70, a lack of ubiquinone occurs due to the missing methylation reaction. The mutant also contains only demethylmenaquinone, no menaquinone. Mutant respiration of DMSO is increased, while the respiration of fumarate is reduced compared to the wild-type enzyme Escherichia coli
metabolism the enzyme catalyzes a step in the menaquinone pathway as well as the methylation step in ubiquinone pathway, overview. Menaquinne activates the respiration of fumarate, trimethylamine N-oxide, nitrate, and DMSO Escherichia coli
physiological function mutant ubiE lacks ubiquinone due to defect in a specific methylation step of ubiquinone synthesis. Synthesis of menaquinone from demethylmenaquinone depends on the same gene ubiE. Mutant contains only demethylmenaquinone, but not menaquinone. Strain is able to grow with fumarate, trimethylamine N-oxide and dimethylsulfoxide, but not with nitrate as electron acceptor. Anaerobic respiration with fumarate and trimethylamine are catalyzed at 69% and 74% of wild-type rates, respectively. Dimethylsulfoxide respiration is reduced to 38% of wild-type, and nitrate respiration is below 8% Escherichia coli