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Literature summary for 2.1.1.161 extracted from
- Identification of glycine betaine as compatible solute in Synechococcus sp. WH8102 and characterization of its N-methyltransferase genes involved in betaine synthesis (2006), Arch. Microbiol., 186, 495-506.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed as soluble His-tag fusion protein in Escherichia coli BL21(DE3)pLysS | Parasynechococcus marenigrum WH 8102 |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.41 | - |
S-adenosyl-L-methionine | pH 8.2, 37°C | Parasynechococcus marenigrum WH 8102 |  |
| 2.11 | - |
N,N-dimethylglycine | pH 8.2, 37°C | Parasynechococcus marenigrum WH 8102 | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
1 * 36000, SDS-PAGE, 1 * 32000, calculated from the deduced amino acid sequence, 1 * 34000, gel fltration | Parasynechococcus marenigrum WH 8102 |
| 34000 | - |
gel filtration | Parasynechococcus marenigrum WH 8102 |
| 36000 | - |
1 * 36000, SDS-PAGE, 1 * 32000, calculated from the deduced amino acid sequence, 1 * 34000, gel fltration | Parasynechococcus marenigrum WH 8102 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + N,N-dimethylglycine | Parasynechococcus marenigrum WH 8102 | synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine | S-adenosyl-L-homocysteine + N,N,N-trimethylglycine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Parasynechococcus marenigrum WH 8102 | Q7U4Z9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein using His-tag | Parasynechococcus marenigrum WH 8102 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | several other amino acids tested as substrates and non of them serves as methyl group acceptor | Parasynechococcus marenigrum WH 8102 | ? | - |
? | |
| S-adenosyl-L-methionine + N,N-dimethylglycine | - |
Parasynechococcus marenigrum WH 8102 | S-adenosyl-L-homocysteine + N,N,N-trimethylglycine | - |
? | |
| S-adenosyl-L-methionine + N,N-dimethylglycine | synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine | Parasynechococcus marenigrum WH 8102 | S-adenosyl-L-homocysteine + N,N,N-trimethylglycine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 36000, SDS-PAGE, 1 * 32000, calculated from the deduced amino acid sequence, 1 * 34000, gel fltration | Parasynechococcus marenigrum WH 8102 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
with N,N-dimethylglycin as substrate | Parasynechococcus marenigrum WH 8102 |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10 | - |
Parasynechococcus marenigrum WH 8102 |
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