Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.160 extracted from

  • McCarthy, A.A.; McCarthy, J.G.
    The structure of two N-methyltransferases from the caffeine biosynthetic pathway (2007), Plant Physiol., 144, 879-889.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.160

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Coffea canephora

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant wild-type and selenomethionine-labeled DXMT, 23-28% PEG 3350, 0.2 M LiCl, 0.1 M Tris-HCl, pH 8.5-8.7, 2 mM DTT, 2 mM S-adenosyl-L-cysteine, and 2 mM theobromine, 1-3 days, 20°C, plate-like crystals, X-ray diffraction structure determination and analysis at 2.0-2.7 A resolution, molecular replacement Coffea canephora

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Coffea canephora

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + 3,7-dimethylxanthine Coffea canephora
-
 S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
-
 ?

Organism

Organism UniProt Comment Textmining
Coffea canephora A4GE70 var. robusta, bifunctional enzyme
-

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + 3,7-dimethylxanthine = S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine residues His160, and Phe266 are involved in catalysis, cosubstrate and substrate binding site structures, overview Coffea canephora
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the bifunctional enzyme also catalyzes the reaction of the theobromine synthase, EC 2.1.1.159 Coffea canephora ?
-
 ?
S-adenosyl-L-methionine + 3,7-dimethylxanthine
-
 Coffea canephora S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
-
 ?
S-adenosyl-L-methionine + 3,7-dimethylxanthine
-
 Coffea canephora S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?

Synonyms

Synonyms Comment Organism
3,7-dimethylxanthine methyltransferase
-
 Coffea canephora
DXMT
-
 Coffea canephora

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Coffea canephora

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
 assay at Coffea canephora