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Literature summary for 2.1.1.160 extracted from

  • Uefuji, H.; Ogita, S.; Yamaguchi, Y.; Koizumi, N.; Sano, H.
    Molecular cloning and functional characterization of three distinct N-methyltransferases involved in the caffeine biosynthetic pathway in coffee plants (2003), Plant Physiol., 132, 372-380.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.160

Cloned(Commentary)

Cloned (Comment) Organism
isozyme DXMT1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of the GST-tagged enzyme in Escherichia coli Bl21(DE3) Coffea arabica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.153
-
 S-adenosyl-L-methionine recombinant DXMT1 Coffea arabica
0.973
-
 paraxanthine recombinant DXMT1 Coffea arabica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43300
-
 2 * 43300, recombinant isozyme DXMT1, SDS-PAGE Coffea arabica
81000
-
 recombinant isozyme DXMT1, gel filtration Coffea arabica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + 3,7-dimethylxanthine Coffea arabica
-
 S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ir

Organism

Organism UniProt Comment Textmining
Coffea arabica Q8H0D2 isozyme DXMT1; isozyme DXMT1
-

Source Tissue

Source Tissue Comment Organism Textmining
flower bud low expression level Coffea arabica
-
fruit immature Coffea arabica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 recombinant enzyme Coffea arabica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the multifunctional isozyme DXMT1 catalyzes also the reaction theobromine synthase, EC 2.1.1.159, but at lower activity compared to its N1-methyltransferase, i.e. caffeine synthase activity Coffea arabica ?
-
 ?
S-adenosyl-L-methionine + 1,7-dimethylxanthine
-
 Coffea arabica S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
-
 ?
S-adenosyl-L-methionine + 3,7-dimethylxanthine
-
 Coffea arabica S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ir

Subunits

Subunits Comment Organism
dimer 2 * 43300, recombinant isozyme DXMT1, SDS-PAGE Coffea arabica

Synonyms

Synonyms Comment Organism
DXMT1
-
 Coffea arabica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
27
-
 assay at Coffea arabica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Coffea arabica