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Literature summary for 2.1.1.160 extracted from

  • McCarthy, A.A.; Biget, L.; Lin, C.; Petiard, V.; Tanksley, S.D.; McCarthy, J.G.
    Cloning, expression, crystallization and preliminary x-ray analysis of the XMT and DXMT N-methyltransferases from Coffea canephora (robusta) (2007), Acta Crystallogr. Sect. F, F63, 304-307.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.160

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged DXMT1 in Escherichia coli strain BL21(DE3) Coffea canephora

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant wild-type and selenomethionine-labeled DXMT, 23-28% PEG 3350, 0.2 M LiCl, 0.1 M Tris-HCl, pH 8.5-8.7, 2 mM DTT, 1 mM S-adenosyl-L-cysteine, and 1 mM theobromine, 1-3 days, 20°C, X-ray diffraction structure determination and analysis at 2.5-2.7 A resolution Coffea canephora

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43400
-
 2 * 43400, about, sequence calculation Coffea canephora
80000
-
 about, recombinant enzyme, gel filtration Coffea canephora

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Coffea canephora the bifunctional enzyme also catalyzes the reaction of the theobromine synthase, EC 2.1.1.159 ?
-
 ?
S-adenosyl-L-methionine + 3,7-dimethylxanthine Coffea canephora
-
 S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?

Organism

Organism UniProt Comment Textmining
Coffea canephora A4GE70 DXMT1; var. robusta, bifunctional enzyme
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged DXMT1 from Escherichia coli strain BL21(DE3) to homogeneity by nickel affinity chromatography, cleavage of the His-tag with tobacco etch virus, TEV, protease, followed by gel filtration Coffea canephora

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the bifunctional enzyme also catalyzes the reaction of the theobromine synthase, EC 2.1.1.159 Coffea canephora ?
-
 ?
S-adenosyl-L-methionine + 3,7-dimethylxanthine
-
 Coffea canephora S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?

Subunits

Subunits Comment Organism
dimer 2 * 43400, about, sequence calculation Coffea canephora

Synonyms

Synonyms Comment Organism
DXMT1
-
 Coffea canephora