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Literature summary for 2.1.1.159 extracted from
- Molecular cloning and functional characterization of three distinct N-methyltransferases involved in the caffeine biosynthetic pathway in coffee plants (2003), Plant Physiol., 132, 372-380.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| isozyme MXMT1, DNA and amnio acid sequence determination and analysis, phylogenetic analysis, expression of the GST-tagged isozymes in Escherichia coli Bl21(DE3) | Coffea arabica |
| isozyme MXMT2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of the GST-tagged isozymes in Escherichia coli Bl21(DE3) | Coffea arabica |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.012 | - |
S-adenosyl-L-methionine | recombinant MXMT1 | Coffea arabica |  |
| 0.014 | - |
S-adenosyl-L-methionine | recombinant MXMT2 | Coffea arabica | |
| 0.148 | - |
7-methylxanthine | recombinant MXMT1 | Coffea arabica | |
| 0.251 | - |
7-methylxanthine | recombinant MXMT2 | Coffea arabica | |
| 0.458 | - |
paraxanthine | recombinant MXMT1 | Coffea arabica | |
| 0.738 | - |
paraxanthine | recombinant MXMT2 | Coffea arabica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43400 | - |
2 * 43400, recombinant isozyme MXMT2, SDS-PAGE | Coffea arabica |
| 74000 | - |
recombinant isozyme MXMT2, gel filtration | Coffea arabica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + 7-methylxanthine | Coffea arabica | - |
S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | i.e. theobromine | ir | |
| S-adenosyl-L-methionine + paraxanthine | Coffea arabica | - |
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | i.e. caffeine | ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Coffea arabica | Q84PP7 | isozyme MXMT2; isozyme MXMT2 | - |
| Coffea arabica | Q9AVJ9 | isozyme MXMT1; isozyme MXTM1 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower bud | isozyme MXMT2 | Coffea arabica | - |
| flower bud | isozyme MXTM1 | Coffea arabica | - |
| fruit | immature, isozyme MXMT2 | Coffea arabica | - |
| fruit | immature, isozyme MXTM1 | Coffea arabica | - |
| leaf | high expression in young leaves of isozyme MXTM2, low expression level in expanded leaves | Coffea arabica | - |
| leaf | very high expression in young leaves of isozyme MXTM1, low expression level in expanded leaves | Coffea arabica | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
recombinant enzyme | Coffea arabica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + 7-methylxanthine | - |
Coffea arabica | S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | i.e. theobromine | ir | |
| S-adenosyl-L-methionine + 7-methylxanthine | preferred substrate, 20fold higher activity by isozyme MXMT2 compared to activity with paraxanthine | Coffea arabica | S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | i.e. theobromine | ir | |
| S-adenosyl-L-methionine + 7-methylxanthine | preferred substrate, 3fold higher activity by isozyme MXMT1 compared to activity with paraxanthine | Coffea arabica | S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | i.e. theobromine | ir | |
| S-adenosyl-L-methionine + paraxanthine | - |
Coffea arabica | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | i.e. caffeine | ir | |
| S-adenosyl-L-methionine + paraxanthine | low activity by isozyme MXMT1 | Coffea arabica | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | i.e. caffeine | ir | |
| S-adenosyl-L-methionine + paraxanthine | low activity by isozyme MXMT2 | Coffea arabica | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | i.e. caffeine | ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 43400, recombinant isozyme MXMT2, SDS-PAGE | Coffea arabica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MXMT1 | - |
Coffea arabica |
| MXMT2 | - |
Coffea arabica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 27 | - |
assay at | Coffea arabica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Coffea arabica |
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