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Literature summary for 2.1.1.159 extracted from

  • Kato, M.; Mizuno, K.; Fujimura, T.; Iwama, M.; Irie, M.; Crozier, A.; Ashihara, H.
    Purification and characterization of caffeine synthase from tea leaves (1999), Plant Physiol., 120, 579-586.
    View publication on PubMedView publication on EuropePMC

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
x * 41000, SDS-PAGE Camellia sinensis
61000
-
gel filtration Camellia sinensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Camellia sinensis the bifunctional enzyme catalyzes the last two steps of the caffeine biosynthesis ?
-
?
S-adenosyl-L-methionine + 3,7-dimethylxanthine Camellia sinensis reaction of EC 2.1.1.160 S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?
S-adenosyl-L-methionine + 7-methylxanthine Camellia sinensis
-
S-adenosyl-L-homocysteine + 3,7-dimethylxanthine i.e. theobromine ?

Organism

Organism UniProt Comment Textmining
Camellia sinensis
-
bifunctional enzyme
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 523fold from young, developing leaves by ammonium sulfate fractionation, hydroxylapatite, anion exchange, and adenosine affinity chromatography, and gel filtration to homogeneity Camellia sinensis

Source Tissue

Source Tissue Comment Organism Textmining
leaf young, developing Camellia sinensis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
95
-
purified enzyme Camellia sinensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the bifunctional enzyme catalyzes the last two steps of the caffeine biosynthesis Camellia sinensis ?
-
?
additional information substrate specificity, overview, the bifunctional enzyme also catalyzes the reaction of the caffeine synthase, EC 2.1.1.160, no activity with xanthosine, xanthosine 5-phosphate, and theophylline Camellia sinensis ?
-
?
S-adenosyl-L-methionine + 1-methylxanthine low activity Camellia sinensis S-adenosyl-L-homocysteine + 1,3-dimethylxanthine i.e. theophylline ?
S-adenosyl-L-methionine + 3,7-dimethylxanthine reaction of EC 2.1.1.160 Camellia sinensis S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?
S-adenosyl-L-methionine + 3-methylxanthine low activity Camellia sinensis S-adenosyl-L-homocysteine + 1,3-dimethylxanthine i.e. theophylline ?
S-adenosyl-L-methionine + 7-methylxanthine
-
Camellia sinensis S-adenosyl-L-homocysteine + 3,7-dimethylxanthine i.e. theobromine ?
S-adenosyl-L-methionine + paraxanthine best substrate Camellia sinensis S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?

Subunits

Subunits Comment Organism
monomer or dimer x * 41000, SDS-PAGE Camellia sinensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
sharp optimum Camellia sinensis

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
pH profile Camellia sinensis