Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression in Escherichia coli as wild-type and as Trx-fusion protein | Coffea arabica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.031 | - |
paraxanthine | pH 8.5, 37°C, recombinant CCS1 | Coffea arabica | |
0.075 | - |
7-methylxanthine | pH 8.5, 37°C, recombinant Trx-fusion CCS1 | Coffea arabica | |
0.126 | - |
7-methylxanthine | pH 8.5, 37°C, recombinant CCS1 | Coffea arabica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Coffea arabica | bifunctional enzyme performing 3-N-methyltransferase activity, EC 2.1.1.159, and 1-N-methyltransferase activity, EC 2.1.1.160, overview | ? | - |
? | |
S-adenosyl-L-methionine + 7-methylxanthine | Coffea arabica | third step in caffeine biosynthesis pathway from xanthosine | S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | i.e. theobromine | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Coffea arabica | Q8H0D3 | bifunctional enzyme performing 3-N-methyltransferase activity, EC 2.1.1.159, and 1-N-methyltransferase activity, EC 2.1.1.160; isozyme CtCS6 is a binfunctional enzyme | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.59 | - |
recombinant CCS1 | Coffea arabica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | bifunctional enzyme performing 3-N-methyltransferase activity, EC 2.1.1.159, and 1-N-methyltransferase activity, EC 2.1.1.160, overview | Coffea arabica | ? | - |
? | |
additional information | substrate specificity, bifunctional enzyme performing 3-N-methyltransferase activity, EC 2.1.1.159, and 1-N-methyltransferase activity, EC 2.1.1.160, overview | Coffea arabica | ? | - |
? | |
S-adenosyl-L-methionine + 7-methylxanthine | - |
Coffea arabica | S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | i.e. theobromine | ? | |
S-adenosyl-L-methionine + 7-methylxanthine | third step in caffeine biosynthesis pathway from xanthosine | Coffea arabica | S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | i.e. theobromine | ? | |
S-adenosyl-L-methionine + paraxanthine | - |
Coffea arabica | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | i.e. caffeine | ? |
Synonyms | Comment | Organism |
---|---|---|
3-N-methyltransferase | - |
Coffea arabica |
caffeine synthase 1 | - |
Coffea arabica |
CCS1 | - |
Coffea arabica |
coffee caffeine synthase 1 | - |
Coffea arabica |
CtCS6 | - |
Coffea arabica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Coffea arabica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Coffea arabica |