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Literature summary for 2.1.1.158 extracted from
- The structure of two N-methyltransferases from the caffeine biosynthetic pathway (2007), Plant Physiol., 144, 879-889.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Coffea canephora |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| purified recombinant wild-type and selenomethionine-labeled XMT, 23-28% PEG 3350, 0.2 M LiCl, 0.1 M Tris-HCl, pH 8.5-8.7, 2 mM DTT, 2 mM S-adenosyl-L-cysteine, and 2 mM xanthosine, 1-3 days, 20°C, plate-like crystals, X-ray diffraction structure determination and analysis at 2.2 A resolution | Coffea canephora |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Coffea canephora |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + xanthosine | Coffea canephora | - |
S-adenosyl-L-homocysteine + 7-methylxanthosine | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Coffea canephora | A4GE69 | var. Robusta | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| S-adenosyl-L-methionine + xanthosine = S-adenosyl-L-homocysteine + 7-methylxanthosine | a catalytic residue is Gln161, cosubstrate and substrate binding site structures involving the Ser316 for xanthosine recognition, overview | Coffea canephora |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + xanthosine | - |
Coffea canephora | S-adenosyl-L-homocysteine + 7-methylxanthosine | - |
ir | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| xanthosine methyltransferase | - |
Coffea canephora |
| XMT | - |
Coffea canephora |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Coffea canephora |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.9 | - |
assay at | Coffea canephora |
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Release 2023.1 (January 2023)
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