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Literature summary for 2.1.1.158 extracted from

  • Mizuno, K.; Kato, M.; Irino, F.; Yoneyama, N.; Fujimura, T.; Ashihara, H.
    The first committed step reaction of caffeine biosynthesis: 7-methylxanthosine synthase is closely homologous to caffeine synthases in coffee (Coffea arabica L.) (2003), FEBS Lett., 547, 56-60.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
gene CtCS1 or CaXMT1 or CmXRS1, DNA sequence determination, phylogenetic tree, expression in Escherichia coli strain BL21(DE3) Coffea arabica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0737
-
 Xanthosine
-
 Coffea arabica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + xanthosine Coffea arabica
-
 S-adenosyl-L-homocysteine + 7-methylxanthosine
-
 ?

Organism

Organism UniProt Comment Textmining
Coffea arabica Q9AVK0 gene CtCS1 or CaXMT1 or CmXRS1
-

Source Tissue

Source Tissue Comment Organism Textmining
endosperm developing Coffea arabica
-
flower bud
-
 Coffea arabica
-
leaf young Coffea arabica
-
additional information quantitative expression analysis Coffea arabica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificity of the recombinant His-tagged enzyme, no activity with xanthosine 5'-phosphate, theobromine, or 7-methylxanthosine Coffea arabica ?
-
 ?
S-adenosyl-L-methionine + xanthosine
-
 Coffea arabica S-adenosyl-L-homocysteine + 7-methylxanthosine
-
 ?
S-adenosyl-L-methionine + xanthosine the enzyme is specific for xanthosine Coffea arabica S-adenosyl-L-homocysteine + 7-methylxanthosine
-
 ?

Synonyms

Synonyms Comment Organism
7-methylxanthine synthase
-
 Coffea arabica
7-methylxanthosine synthase 1
-
 Coffea arabica
CmXRS1
-
 Coffea arabica
xanthosine methyltransferase
-
 Coffea arabica
xanthosine:S-adenosyl-L-methionine methyltransferase
-
 Coffea arabica