Literature summary for 2.1.1.158 extracted from

McCarthy, A.A.; Biget, L.; Lin, C.; Petiard, V.; Tanksley, S.D.; McCarthy, J.G.
Cloning, expression, crystallization and preliminary x-ray analysis of the XMT and DXMT N-methyltransferases from Coffea canephora (robusta) (2007), Acta Crystallogr. Sect. F, F63, 304-307.

Search for more literature for 2.1.1.158

Cloned(Commentary)

Cloned (Comment)	Organism
expression of His-tagged XMT1 in Escherichia coli strain BL21(DE3)	Coffea canephora

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant wild-type and selenomethionine-labeled XMT, 23-28% PEG 3350, 0.2 M LiCl, 0.1 M Tris-HCl, pH 8.5-8.7, 2 mM DTT, 1 mM S-adenosyl-L-cysteine, and 1 mM xanthosine, 1-3 days, 20°C, X-ray diffraction structure determination and analysis at 2.8-3.0 A resolution	Coffea canephora

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
42000	-	2 * 42000, about, sequence calculation	Coffea canephora
80000	-	about, recombinant enzyme, gel filtration	Coffea canephora

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + xanthosine	Coffea canephora	-	S-adenosyl-L-homocysteine + 7-methylxanthosine	-	ir

Organism

Organism	UniProt	Comment	Textmining
Coffea canephora	A4GE69	XMT1; var. Robusta	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged XMT1 from Escherichia coli strain BL21(DE3) to homogeneity by nickel affinity chromatography, cleavage of the His-tag with tobacco etch virus, TEV, protease, followed by gel filtration	Coffea canephora

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + xanthosine	-	Coffea canephora	S-adenosyl-L-homocysteine + 7-methylxanthosine	-	ir

Subunits

Subunits	Comment	Organism
dimer	2 * 42000, about, sequence calculation	Coffea canephora

Synonyms

Synonyms	Comment	Organism
XMT1	-	Coffea canephora

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Release 2023.1 (January 2023)