Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.154 extracted from

  • Maxwell, C.A.; Edwards, R.; Dixon, R.A.
    Identification, purification, and characterization of S-adenosyl-L-methionine: isoliquiritigenin 2'-O-methyltransferase from alfalfa (Medicago sativa L.) (1992), Arch. Biochem. Biophys., 293, 158-166.
    View publication on PubMed

General Stability

General Stability Organism
stabilized in presence of 5 mM EDTA Medicago sativa

Inhibitors

Inhibitors Comment Organism Structure
4,4'-dihydroxy-2'-methoxychalcone noncompetitive Medicago sativa
Co2+ 1 mM, 22% inhibition Medicago sativa
Cu2+ 1 mM, 12% inhibition Medicago sativa
Fe2+ 1 mM, 44% inhibition Medicago sativa
S-adenosyl-L-homocysteine competitive with respect to S-adenosyl-L-methionine Medicago sativa
Zn2+ 1 mM, 45% inhibition Medicago sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2
-
isoliquiritigenin
-
Medicago sativa
17.7
-
S-adenosyl-L-methionine
-
Medicago sativa

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble 98% of the activity is in the soluble fraction Medicago sativa
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
1 * 43000, SDS-PAGE Medicago sativa
57000
-
gel filtration Medicago sativa

Organism

Organism UniProt Comment Textmining
Medicago sativa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Medicago sativa

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + isoliquiritigenin = S-adenosyl-L-homocysteine + 2'-O-methylisoliquiritigenin sequential Bi Bi mechanism Medicago sativa

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Medicago sativa
-
root enzyme activity increases during early stages of seedling development and is predomonantly located in the root Medicago sativa
-
seedling enzyme activity increases during early stages of seedling development and is predomonantly located in the root Medicago sativa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.13
-
-
Medicago sativa

Storage Stability

Storage Stability Organism
-80°C, 12% loss of activity after 1.5 months Medicago sativa
4°C, half-life: 3-4 days Medicago sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity towards naringenin chalcone, caffeic acid or daidzein Medicago sativa ?
-
?
S-adenosyl-L-methionine + 2',4'-dihydroxy-4-methoxychalcone 77% of the activity with isoliquiritigenin Medicago sativa S-adenosyl-L-homocysteine + 4'-hydroxy-2',4-dimethoxychalcone
-
?
S-adenosyl-L-methionine + 4',7-dihydroxyflavanone 32% of the activity with isoliquiritigenin Medicago sativa S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + isoliquiritigenin
-
Medicago sativa S-adenosyl-L-homocysteine + 2'-O-methylisoliquiritigenin
-
?

Subunits

Subunits Comment Organism
monomer 1 * 43000, SDS-PAGE Medicago sativa

Synonyms

Synonyms Comment Organism
chalcone OMT
-
Medicago sativa
CHMT
-
Medicago sativa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
half-life: 3-4 days Medicago sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Medicago sativa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0022
-
S-adenosyl-L-homocysteine
-
Medicago sativa
0.0652
-
4,4'-dihydroxy-2'-methoxychalcone
-
Medicago sativa

pI Value

Organism Comment pI Value Maximum pI Value
Medicago sativa chromatofocusing
-
4.9