BRENDA - Enzyme Database
show all sequences of 2.1.1.146

Characterization of phenylpropene O-methyltransferases from sweet basil: facile change of substrate specificity and convergent evolution within a plant O-methyltransferase family

Gang, D.R.; Lavid, N.; Zubieta, C.; Chen, F.; Beuerle, T.; Lewinsohn, E.; Noel, J.P.; Pichersky, E.; Plant Cell 14, 505-519 (2002)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
-
Ocimum basilicum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.003
-
eugenol
-
Ocimum basilicum
0.005
-
S-adenosyl-L-methionine
-
Ocimum basilicum
0.007
-
chavicol
-
Ocimum basilicum
0.01
-
trans-isoeugenol
-
Ocimum basilicum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
2 * 40000, SDS-PAGE
Ocimum basilicum
81000
-
gel filtration
Ocimum basilicum
Organism
Organism
UniProt
Commentary
Textmining
Ocimum basilicum
Q93WU2
variety EMX-1
-
Purification (Commentary)
Purification (Commentary)
Organism
partial
Ocimum basilicum
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
young
Ocimum basilicum
-
trichome
-
Ocimum basilicum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
S-adenosyl-L-methionine + chavicol
24% of the activity with eugenol
485155
Ocimum basilicum
S-adenosyl-L-homocysteine + estragol
-
-
-
?
S-adenosyl-L-methionine + eugenol
-
485155
Ocimum basilicum
S-adenosyl-L-homocysteine + methyleugenol
-
-
-
?
S-adenosyl-L-methionine + guaiacol
29.9% of the activity with eugenol
485155
Ocimum basilicum
S-adenosyl-L-homocysteine + 1,2-dimethoxybenzene
-
-
-
?
S-adenosyl-L-methionine + isoeugenol
26% of the activity with eugenol
485155
Ocimum basilicum
S-adenosyl-L-homocysteine + isomethyleugenol
-
485155
Ocimum basilicum
?
S-adenosyl-L-methionine + trans-isoeugenol
-
485155
Ocimum basilicum
S-adenosyl-L-homocysteine + (E)-2',3'-dimethoxy-1-propenylbenzene
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 40000, SDS-PAGE
Ocimum basilicum
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.00087
-
chavicol
-
Ocimum basilicum
0.0013
-
t-isoeugenol
-
Ocimum basilicum
0.0031
-
eugenol
-
Ocimum basilicum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
8
-
Ocimum basilicum
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Ocimum basilicum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.003
-
eugenol
-
Ocimum basilicum
0.005
-
S-adenosyl-L-methionine
-
Ocimum basilicum
0.007
-
chavicol
-
Ocimum basilicum
0.01
-
trans-isoeugenol
-
Ocimum basilicum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
2 * 40000, SDS-PAGE
Ocimum basilicum
81000
-
gel filtration
Ocimum basilicum
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Ocimum basilicum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
young
Ocimum basilicum
-
trichome
-
Ocimum basilicum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
S-adenosyl-L-methionine + chavicol
24% of the activity with eugenol
485155
Ocimum basilicum
S-adenosyl-L-homocysteine + estragol
-
-
-
?
S-adenosyl-L-methionine + eugenol
-
485155
Ocimum basilicum
S-adenosyl-L-homocysteine + methyleugenol
-
-
-
?
S-adenosyl-L-methionine + guaiacol
29.9% of the activity with eugenol
485155
Ocimum basilicum
S-adenosyl-L-homocysteine + 1,2-dimethoxybenzene
-
-
-
?
S-adenosyl-L-methionine + isoeugenol
26% of the activity with eugenol
485155
Ocimum basilicum
S-adenosyl-L-homocysteine + isomethyleugenol
-
485155
Ocimum basilicum
?
S-adenosyl-L-methionine + trans-isoeugenol
-
485155
Ocimum basilicum
S-adenosyl-L-homocysteine + (E)-2',3'-dimethoxy-1-propenylbenzene
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 40000, SDS-PAGE
Ocimum basilicum
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.00087
-
chavicol
-
Ocimum basilicum
0.0013
-
t-isoeugenol
-
Ocimum basilicum
0.0031
-
eugenol
-
Ocimum basilicum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
8
-
Ocimum basilicum
Other publictions for EC 2.1.1.146
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
734667
Renu
Characterization and functiona ...
Ocimum tenuiflorum
Mol. Biol. Rep.
41
1857-1870
2014
-
-
-
-
-
-
-
-
-
-
1
1
-
2
-
-
-
-
-
1
-
-
1
1
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
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-
-
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-
-
-
-
1
1
-
-
-
-
-
1
-
-
1
1
-
-
-
-
-
-
-
1
1
-
-
1
-
-
733041
Tarchoune
-
Effects of two sodium salts on ...
Ocimum basilicum
Acta Physiol. Plant.
35
2365-2372
2013
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-
-
-
-
-
-
-
-
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1
-
1
-
-
-
-
-
1
-
-
1
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1
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1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
700822
Koeduka
Biosynthesis of t-anethole in ...
Pimpinella anisum
Plant Physiol.
149
384-394
2009
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-
1
-
-
-
5
2
-
-
-
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-
7
-
-
-
-
-
2
-
-
6
-
1
-
-
4
1
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
5
-
2
-
-
-
-
-
-
-
-
-
2
-
-
6
-
-
-
4
1
1
1
-
-
-
-
-
-
1
1
659534
Wu
Two O-methyltransferases isola ...
Rosa chinensis
J. Biosci. Bioeng.
96
119-128
2003
-
-
1
-
-
-
-
2
-
-
-
-
-
3
-
-
-
-
-
-
-
-
5
-
1
-
-
-
2
-
-
-
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-
-
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-
-
1
-
-
-
-
-
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-
2
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-
-
5
-
-
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-
2
-
-
-
-
-
-
-
-
-
-
659911
Barkman
Evidence for positive selectio ...
Clarkia breweri
Mol. Biol. Evol.
20
168-172
2003
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
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-
-
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-
-
2
-
-
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-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485155
Gang
Characterization of phenylprop ...
Ocimum basilicum
Plant Cell
14
505-519
2002
-
-
1
-
-
-
-
4
-
-
2
-
-
5
-
-
1
-
-
2
-
-
5
1
-
-
-
-
3
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
4
-
-
2
-
-
-
-
1
-
2
-
-
5
1
-
-
-
3
1
-
-
-
-
-
-
-
-
-
485153
Wang
Characterization of S-adenosyl ...
Clarkia breweri
Arch. Biochem. Biophys.
349
153-160
1998
-
-
1
-
-
-
10
6
-
1
2
-
-
4
-
-
1
-
-
2
1
-
2
1
-
-
-
2
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
10
-
6
-
1
2
-
-
-
-
1
-
2
1
-
2
1
-
-
2
-
1
-
-
-
-
-
-
-
-
-
485154
Wang
Floral scent production in Cla ...
Clarkia breweri
Plant Physiol.
114
213-221
1997
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
3
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-