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Literature summary for 2.1.1.146 extracted from
- Characterization of S-adenosyl-L-methionine:(iso)eugenol O-methyltransferase involved in floral scent production in Clarkia breweri (1998), Arch. Biochem. Biophys., 349, 153-160.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Clarkia breweri |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 5 mM, weak inhibition | Clarkia breweri |  |
| Cu2+ | 5 mM, strong inhibition | Clarkia breweri | |
| Fe2+ | 5 mM, strong inhibition | Clarkia breweri | |
| Fe3+ | 5 mM, strong inhibition | Clarkia breweri | |
| K+ | 5 mM, weak inhibition | Clarkia breweri | |
| Mg2+ | 5 mM, 33% inhibition | Clarkia breweri | |
| Mn2+ | 5 mM, strong inhibition | Clarkia breweri | |
| Na+ | 5 mM, weak inhibition | Clarkia breweri | |
| NH4+ | 5 mM, weak inhibition | Clarkia breweri | |
| Zn2+ | 5 mM, strong inhibition | Clarkia breweri | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.007 | - |
eugenol | plant-purified enzyme | Clarkia breweri | |
| 0.019 | - |
S-adenosyl-L-methionine | recombinant enzyme | Clarkia breweri | |
| 0.027 | - |
S-adenosyl-L-methionine | plant-purified enzyme | Clarkia breweri | |
| 0.03 | - |
eugenol | recombinant enzyme | Clarkia breweri | |
| 0.058 | - |
isoeugenol | plant-purified enzyme | Clarkia breweri | |
| 0.074 | - |
isoeugenol | recombinant enzyme | Clarkia breweri | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | methylation of eugenol and isoeugenol | Clarkia breweri |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
2 * 40000, SDS-PAGE | Clarkia breweri |
| 88000 | - |
gel filtration | Clarkia breweri |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clarkia breweri | O04385 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Clarkia breweri |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower | - |
Clarkia breweri | - |
| petal | - |
Clarkia breweri | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Clarkia breweri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + eugenol | - |
Clarkia breweri | S-adenosyl-L-homocysteine + methyleugenol | - |
? | |
| S-adenosyl-L-methionine + isoeugenol | - |
Clarkia breweri | S-adenosyl-L-homocysteine + isomethyleugenol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 40000, SDS-PAGE | Clarkia breweri |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
30 min, stable up to | Clarkia breweri |
| 45 | - |
30 min, activity is dramatically decreased | Clarkia breweri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
methylation of eugenol and isoeugenol | Clarkia breweri |
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