BRENDA - Enzyme Database
show all sequences of 2.1.1.146

Characterization of S-adenosyl-L-methionine:(iso)eugenol O-methyltransferase involved in floral scent production in Clarkia breweri

Wang, J.; Pichersky, E.; Arch. Biochem. Biophys. 349, 153-160 (1998)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Clarkia breweri
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ca2+
5 mM, weak inhibition
Clarkia breweri
Cu2+
5 mM, strong inhibition
Clarkia breweri
Fe2+
5 mM, strong inhibition
Clarkia breweri
Fe3+
5 mM, strong inhibition
Clarkia breweri
K+
5 mM, weak inhibition
Clarkia breweri
Mg2+
5 mM, 33% inhibition
Clarkia breweri
Mn2+
5 mM, strong inhibition
Clarkia breweri
Na+
5 mM, weak inhibition
Clarkia breweri
NH4+
5 mM, weak inhibition
Clarkia breweri
Zn2+
5 mM, strong inhibition
Clarkia breweri
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.007
-
eugenol
plant-purified enzyme
Clarkia breweri
0.019
-
S-adenosyl-L-methionine
recombinant enzyme
Clarkia breweri
0.027
-
S-adenosyl-L-methionine
plant-purified enzyme
Clarkia breweri
0.03
-
eugenol
recombinant enzyme
Clarkia breweri
0.058
-
isoeugenol
plant-purified enzyme
Clarkia breweri
0.074
-
isoeugenol
recombinant enzyme
Clarkia breweri
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
methylation of eugenol and isoeugenol
Clarkia breweri
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
2 * 40000, SDS-PAGE
Clarkia breweri
88000
-
gel filtration
Clarkia breweri
Organism
Organism
UniProt
Commentary
Textmining
Clarkia breweri
O04385
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Clarkia breweri
Source Tissue
Source Tissue
Commentary
Organism
Textmining
flower
-
Clarkia breweri
-
petal
-
Clarkia breweri
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Clarkia breweri
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
S-adenosyl-L-methionine + eugenol
-
485153
Clarkia breweri
S-adenosyl-L-homocysteine + methyleugenol
-
485153
Clarkia breweri
?
S-adenosyl-L-methionine + isoeugenol
-
485153
Clarkia breweri
S-adenosyl-L-homocysteine + isomethyleugenol
-
485153
Clarkia breweri
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 40000, SDS-PAGE
Clarkia breweri
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
35
-
30 min, stable up to
Clarkia breweri
45
-
30 min, activity is dramatically decreased
Clarkia breweri
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
methylation of eugenol and isoeugenol
Clarkia breweri
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Clarkia breweri
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ca2+
5 mM, weak inhibition
Clarkia breweri
Cu2+
5 mM, strong inhibition
Clarkia breweri
Fe2+
5 mM, strong inhibition
Clarkia breweri
Fe3+
5 mM, strong inhibition
Clarkia breweri
K+
5 mM, weak inhibition
Clarkia breweri
Mg2+
5 mM, 33% inhibition
Clarkia breweri
Mn2+
5 mM, strong inhibition
Clarkia breweri
Na+
5 mM, weak inhibition
Clarkia breweri
NH4+
5 mM, weak inhibition
Clarkia breweri
Zn2+
5 mM, strong inhibition
Clarkia breweri
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.007
-
eugenol
plant-purified enzyme
Clarkia breweri
0.019
-
S-adenosyl-L-methionine
recombinant enzyme
Clarkia breweri
0.027
-
S-adenosyl-L-methionine
plant-purified enzyme
Clarkia breweri
0.03
-
eugenol
recombinant enzyme
Clarkia breweri
0.058
-
isoeugenol
plant-purified enzyme
Clarkia breweri
0.074
-
isoeugenol
recombinant enzyme
Clarkia breweri
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
methylation of eugenol and isoeugenol
Clarkia breweri
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
2 * 40000, SDS-PAGE
Clarkia breweri
88000
-
gel filtration
Clarkia breweri
Purification (Commentary) (protein specific)
Commentary
Organism
-
Clarkia breweri
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
flower
-
Clarkia breweri
-
petal
-
Clarkia breweri
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Clarkia breweri
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
S-adenosyl-L-methionine + eugenol
-
485153
Clarkia breweri
S-adenosyl-L-homocysteine + methyleugenol
-
485153
Clarkia breweri
?
S-adenosyl-L-methionine + isoeugenol
-
485153
Clarkia breweri
S-adenosyl-L-homocysteine + isomethyleugenol
-
485153
Clarkia breweri
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 40000, SDS-PAGE
Clarkia breweri
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
35
-
30 min, stable up to
Clarkia breweri
45
-
30 min, activity is dramatically decreased
Clarkia breweri
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
methylation of eugenol and isoeugenol
Clarkia breweri
Other publictions for EC 2.1.1.146
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
734667
Renu
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Ocimum tenuiflorum
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1
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2
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1
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1
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1
1
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1
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1
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733041
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2365-2372
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1
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1
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1
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1
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1
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700822
Koeduka
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Pimpinella anisum
Plant Physiol.
149
384-394
2009
-
-
1
-
-
-
5
2
-
-
-
-
-
7
-
-
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-
-
2
-
-
6
-
1
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4
1
1
1
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1
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5
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2
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2
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-
6
-
-
-
4
1
1
1
-
-
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-
-
1
1
659534
Wu
Two O-methyltransferases isola ...
Rosa chinensis
J. Biosci. Bioeng.
96
119-128
2003
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-
1
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2
-
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-
3
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5
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1
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2
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1
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2
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-
-
5
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
659911
Barkman
Evidence for positive selectio ...
Clarkia breweri
Mol. Biol. Evol.
20
168-172
2003
-
-
-
-
-
-
-
-
-
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2
-
2
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4
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2
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-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485155
Gang
Characterization of phenylprop ...
Ocimum basilicum
Plant Cell
14
505-519
2002
-
-
1
-
-
-
-
4
-
-
2
-
-
5
-
-
1
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2
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5
1
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3
1
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-
1
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4
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2
-
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1
-
2
-
-
5
1
-
-
-
3
1
-
-
-
-
-
-
-
-
-
485153
Wang
Characterization of S-adenosyl ...
Clarkia breweri
Arch. Biochem. Biophys.
349
153-160
1998
-
-
1
-
-
-
10
6
-
1
2
-
-
4
-
-
1
-
-
2
1
-
2
1
-
-
-
2
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
10
-
6
-
1
2
-
-
-
-
1
-
2
1
-
2
1
-
-
2
-
1
-
-
-
-
-
-
-
-
-
485154
Wang
Floral scent production in Cla ...
Clarkia breweri
Plant Physiol.
114
213-221
1997
-
-
1
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-
-
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-
4
-
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-
3
-
-
2
-
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-
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-
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1
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-
3
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2
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