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Literature summary for 2.1.1.142 extracted from

  • Neelakandan, A.K.; Song, Z.; Wang, J.; Richards, M.H.; Wu, X.; Valliyodan, B.; Nguyen, H.T.; Nes, W.D.
    Cloning, functional expression and phylogenetic analysis of plant sterol 24C-methyltransferases involved in sitosterol biosynthesis (2009), Phytochemistry, 70, 1982-1998.
    View publication on PubMed
Search for more literature for 2.1.1.142

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Glycine max

Inhibitors

Inhibitors Comment Organism Structure
24(28)-methylene cycloartanol competitive Glycine max
25-azacycloartanol non-competitive Glycine max
25-azacyloartenol
-
 Glycine max
sitosterol competitive Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
 Cycloartenol
-
 Glycine max
0.028
-
 24(28)methylene lophenol
-
 Glycine max
0.03
-
 Cycloartenol
-
 Glycine max
0.03
-
 24(28)-methylene lophenol
-
 Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 4 * 40340, calculated, 4 * 40000, SDS-PAGE Glycine max
40340
-
 4 * 40340, calculated, 4 * 40000, SDS-PAGE Glycine max
40397
-
 x * 40397, calculated Glycine max
160000
-
 gel filtration Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max
-
 isoform SMT2-1
-
Glycine max D2D5G4 isoform SMT2-2
-
Glycine max Q43445
-
-

Purification (Commentary)

Purification (Comment) Organism
isoform SMT2-2 is purified to homogeneity by column chromatography, gel filtration Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Glycine max
-
leaf young leaf Glycine max
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
24(28)-methylene lophenol + S-adenosyl-L-methionine
-
 Glycine max ?
-
 ?
24(28)-methylene lophenol + S-adenosyl-L-methionine
-
 Glycine max 24(28)Z-ethylidene lophenol + S-adenosyl-L-homocysteine
-
 ?
cycloartenol + S-adenosyl-L-methionine
-
 Glycine max 24-methylenecycloartanol + S-adenosyl-L-homocysteine
-
 ?
cycloartenol + S-adenosyl-L-methionine 64% of the effectiveness with 24(28)-methylene lophenol Glycine max 24-methylenecycloartanol + S-adenosyl-L-homocysteine
-
 ?
cycloartenol + S-adenosyl-L-methionine optimal substrate for SMT1 Glycine max 24(28)-methylene cycloartanol + S-adenosyl-L-homocysteine
-
 ?
ergosta-7,24(28)-dienol + S-adenosyl-L-methionine fecosterol and ergosta-7,24(28)-dienol which lack C4-and C14-methyl groups in the nucleus are catalyzed equally effectively by SMT2-2 as the natural substrate 24(28)-methylene lophenol Glycine max ? + S-adenosyl-L-homocysteine
-
 ?
fecosterol + S-adenosyl-L-methionine fecosterol and ergosta-7,24(28)-dienol which lack C4-and C14-methyl groups in the nucleus are catalyzed equally effectively by SMT2-2 as the natural substrate 24(28)-methylene lophenol Glycine max ? + S-adenosyl-L-homocysteine
-
 ?
lanosterol + S-adenosyl-L-methionine
-
 Glycine max ? + S-adenosyl-L-homocysteine
-
 ?
zymosterol + S-adenosyl-L-methionine
-
 Glycine max fecosterol + S-adenosyl-L-homocysteine
-
 ?

Subunits

Subunits Comment Organism
? x * 40397, calculated Glycine max
tetramer 4 * 40340, calculated, 4 * 40000, SDS-PAGE Glycine max

Synonyms

Synonyms Comment Organism
SMT1
-
 Glycine max
sterol 24C-methyltransferases
-
 Glycine max
zymosterol-24(25)-methyltransferase
-
 Glycine max

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.01
-
 Cycloartenol
-
 Glycine max
0.013
-
 24(28)methylene lophenol
-
 Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Glycine max

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Glycine max

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00001
-
 25-azacycloartanol substrate cycloartenol Glycine max
0.00002
-
 25-azacycloartanol substrate 24(28)-methylene lophenol Glycine max
0.00004
-
 25-azacyloartenol
-
 Glycine max
0.00009
-
 24(28)-methylene cycloartanol
-
 Glycine max
0.0001
-
 sitosterol
-
 Glycine max
0.077
-
 24(28)-methylene cycloartanol substrate cycloartenol Glycine max
0.1
-
 sitosterol substrate cycloartenol Glycine max
0.116
-
 24(28)-methylene cycloartanol substrate 24(28)-methylene lophenol Glycine max
0.154
-
 sitosterol substrate 24(28)-methylene lophenol Glycine max

pI Value

Organism Comment pI Value Maximum pI Value
Glycine max calculated
-
 6.3
Glycine max calculated
-
 6.5