Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli from the Salmonella enterica prpBCDE promoter, comparison to expression products derived from the strongest IPTG-inducible promoter PT7, DH10B strain used for DNA-manipulations, BLRDE3 strain host for pPro and T7 expression systems | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
recombinant protein, gel filtration and SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + (S)-coclaurine | Escherichia coli | isoquinoline alkaloid biosynthesis | S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein, gel filtration | Escherichia coli |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture supernatant | - |
Escherichia coli | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
analysis of inducible expression systems, production of recombinant CMT protein from PprpB promoter 2fold to 4fold higher than that from PT7 promoter, cell growth not reduced after expression form PprpB promoter even when highly induced, propionate-inducible system efficient for protein overproduction that result in growth inhibition, auto-induction experiments performed and protein production monitored | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + (S)-coclaurine | isoquinoline alkaloid biosynthesis | Escherichia coli | S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine | - |
? | |
S-adenosyl-L-methionine + (S)-coclaurine | expression systems tested by analyzing recombinant CMT protein | Escherichia coli | S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CMT | - |
Escherichia coli |
coclaurine N-methyltransferase | - |
Escherichia coli |