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Literature summary for 2.1.1.140 extracted from

  • Choi, K.B.; Morishige, T.; Shitan, N.; Yazaki, K.; Sato, F.
    Molecular cloning and characterization of coclaurine N-methyltransferase from cultured cells of Coptis japonica (2002), J. Biol. Chem., 277, 830-835.
    View publication on PubMed
Search for more literature for 2.1.1.140

Application

Application Comment Organism
biotechnology CNMT should be quite useful for biotransformation of the intermediates of alkaloid biosynthesis to N-methyltransferred products Coptis japonica

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Coptis japonica

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 5 mM, 37% inhibition Coptis japonica
Cu2+ 5 mM, 52% inhibition Coptis japonica
Mn2+ 5 mM, 52% inhibition Coptis japonica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
 (R,S)-norreticuline recombinant CNMT Coptis japonica
0.39
-
 S-adenosyl-L-methionine recombinant CNMT Coptis japonica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + (S)-coclaurine Coptis japonica isoquinoline alkaloid biosynthesis S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?

Organism

Organism UniProt Comment Textmining
Coptis japonica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Coptis japonica

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Coptis japonica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.08
-
-
 Coptis japonica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with: (R,S)-scoulerine, 1,2,3,4-tetrahydroisoquinoline and 1,2,3,4-tetrahydro-3-isoquinoline carboxylic acid (+)-emetine Coptis japonica ?
-
 ?
S-adenosyl-L-methionine + (R)-coclaurine 240% of the activity with (R,S)-norreticuline Coptis japonica S-adenosyl-L-homocysteine + (R)-N-methylcoclaurine
-
 ?
S-adenosyl-L-methionine + (R,S)-6-O-methylnorlaudanosoline 69% of the activity with (R,S)-norreticuline Coptis japonica S-adenosyl-L-homocysteine + (R,S)-N-methyl-6-O-methylnorlaudanosoline
-
 ?
S-adenosyl-L-methionine + (R,S)-norlaudanosoline 49% of the activity with (R,S)-norreticuline Coptis japonica S-adenosyl-L-homocysteine + (R,S)-N-methylnorlaudanosoline
-
 ?
S-adenosyl-L-methionine + (S)-coclaurine isoquinoline alkaloid biosynthesis Coptis japonica S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?
S-adenosyl-L-methionine + (S)-coclaurine sequential mechanism, 153% of the activity with (R,S)-norreticuline Coptis japonica S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?
S-adenosyl-L-methionine + 1-methyl-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline 15% of the activity with (R,S)-norreticuline Coptis japonica S-adenosyl-L-homocysteine + 6,7-dihydroxy-1,N-dimethyl-1,2,3,4-tetrahydroisoquinoline
-
 ?
S-adenosyl-L-methionine + 6,7-dimethoxy-N-methyl-1,2,3,4-tetrahydroisoquinoline 180% of the activity with (R,S)-norreticuline Coptis japonica S-adenosyl-L-homocysteine + 6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline
-
 ?

Synonyms

Synonyms Comment Organism
CNMT
-
 Coptis japonica
coclaurine N-methyltransferase
-
 Coptis japonica
S-adenosyl-L-methionine:coclaurine N-methyltransferase
-
 Coptis japonica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Coptis japonica

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8 about 50% of maximal activity at pH 6.5 and at pH 8.0 Coptis japonica