Inhibitors | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | 50% inhibition at 0.062 mM, substrate (S)-coclaurine | Tinospora cordifolia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
S-adenosyl-L-methionine | - |
Tinospora cordifolia | |
0.04 | - |
(S)-Coclaurine | - |
Tinospora cordifolia | |
0.05 | - |
(S)-norcoclaurine | probably allosteric enzyme, Hill coeffizient 2.6 | Tinospora cordifolia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
83000 | - |
gel filtration HPLC | Tinospora cordifolia |
87000 | - |
gel filtration | Tinospora cordifolia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-coclaurine + S-adenosyl-L-methionine | Eschscholzia californica | - |
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine | - |
? | |
(S)-coclaurine + S-adenosyl-L-methionine | Lamprocapnos spectabilis | - |
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine | - |
? | |
(S)-coclaurine + S-adenosyl-L-methionine | Fumaria officinalis | - |
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine | - |
? | |
(S)-coclaurine + S-adenosyl-L-methionine | Berberis koetineana | - |
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine | - |
? | |
(S)-coclaurine + S-adenosyl-L-methionine | Berberis stolonifera | - |
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine | - |
? | |
(S)-coclaurine + S-adenosyl-L-methionine | Bocconia cordata | - |
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine | - |
? | |
(S)-coclaurine + S-adenosyl-L-methionine | Corydalis vaginans | - |
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine | - |
? | |
(S)-coclaurine + S-adenosyl-L-methionine | Stephania delavayi | - |
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine | - |
? | |
(S)-coclaurine + S-adenosyl-L-methionine | Tinospora cordifolia | - |
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine | - |
? | |
(S)-coclaurine + S-adenosyl-L-methionine | Berberis canadensis | - |
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine | - |
? | |
(S)-coclaurine + S-adenosyl-L-methionine | Cocculus trilobus | - |
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine | - |
? | |
(S)-coclaurine + S-adenosyl-L-methionine | Tinospora fragosa | - |
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine | - |
? | |
(S)-coclaurine + S-adenosyl-L-methionine | Thalictrum dasycarpum | - |
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine | - |
? | |
(S)-coclaurine + S-adenosyl-L-methionine | Thalictrum flavum | - |
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine | - |
? | |
(S)-coclaurine + S-adenosyl-L-methionine | Thalictrum glaucum | - |
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Berberis canadensis | - |
- |
- |
Berberis koetineana | - |
- |
- |
Berberis stolonifera | - |
- |
- |
Bocconia cordata | - |
- |
- |
Cocculus trilobus | - |
- |
- |
Corydalis vaginans | - |
- |
- |
Eschscholzia californica | - |
- |
- |
Fumaria officinalis | - |
- |
- |
Lamprocapnos spectabilis | - |
- |
- |
Stephania delavayi | - |
- |
- |
Thalictrum dasycarpum | - |
- |
- |
Thalictrum flavum | - |
- |
- |
Thalictrum glaucum | - |
- |
- |
Tinospora cordifolia | - |
- |
- |
Tinospora fragosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
40% ammonium sulfate, Phenyl Sepharose, TSK Fraktogel, Mono Q, Affinity gel, disc electrophoresis, 115fold purification | Tinospora cordifolia |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000096 | - |
enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated | Lamprocapnos spectabilis |
0.000096 | - |
enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated | Fumaria officinalis |
0.0001 | - |
enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated | Stephania delavayi |
0.0003 | - |
enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated | Thalictrum flavum |
0.00065 | - |
enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated | Thalictrum glaucum |
0.00066 | - |
enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated | Thalictrum dasycarpum |
0.0007 | - |
enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated | Corydalis vaginans |
0.00075 | - |
enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated | Bocconia cordata |
0.00077 | - |
enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated | Cocculus trilobus |
0.0008 | - |
enzyme extract of cell culture, (S)-coclaurine | Berberis stolonifera |
0.0013 | - |
enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated | Eschscholzia californica |
0.002 | - |
enzyme extract of cell culture, (S)-coclaurine | Berberis koetineana |
0.002 | - |
enzyme extract of cell culture, (S)-coclaurine | Berberis canadensis |
0.003 | - |
enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated | Tinospora fragosa |
0.0036 | - |
enzyme extract of cell culture, (S)-coclaurine | Tinospora cordifolia |
0.4 | - |
- |
Tinospora cordifolia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-coclaurine + S-adenosyl-L-methionine | - |
Eschscholzia californica | S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine | - |
? | |
(S)-coclaurine + S-adenosyl-L-methionine | - |
Lamprocapnos spectabilis | S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine | - |
? | |
(S)-coclaurine + S-adenosyl-L-methionine | - |
Fumaria officinalis | S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine | - |
? | |
(S)-coclaurine + S-adenosyl-L-methionine | - |
Berberis koetineana | S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine | - |
? | |
(S)-coclaurine + S-adenosyl-L-methionine | - |
Berberis stolonifera | S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine | - |
? | |
(S)-coclaurine + S-adenosyl-L-methionine | - |
Bocconia cordata | S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine | - |
? | |
(S)-coclaurine + S-adenosyl-L-methionine | - |
Corydalis vaginans | S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine | - |
? | |
(S)-coclaurine + S-adenosyl-L-methionine | - |
Stephania delavayi | S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine | - |
? | |
(S)-coclaurine + S-adenosyl-L-methionine | - |
Tinospora cordifolia | S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine | - |
? | |
(S)-coclaurine + S-adenosyl-L-methionine | - |
Berberis canadensis | S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine | - |
? | |
(S)-coclaurine + S-adenosyl-L-methionine | - |
Cocculus trilobus | S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine | - |
? | |
(S)-coclaurine + S-adenosyl-L-methionine | - |
Tinospora fragosa | S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine | - |
? | |
(S)-coclaurine + S-adenosyl-L-methionine | - |
Thalictrum dasycarpum | S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine | - |
? | |
(S)-coclaurine + S-adenosyl-L-methionine | - |
Thalictrum flavum | S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine | - |
? | |
(S)-coclaurine + S-adenosyl-L-methionine | - |
Thalictrum glaucum | S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine | - |
? | |
(S)-coclaurine + S-adenosyl-L-methionine | strictly stereospecific, only (S)-coclaurine is N-methylated | Tinospora cordifolia | S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine | - |
? | |
(S)-norcoclaurine + S-adenosyl-L-methionine | strictly stereospecific, only (S)-norcoclaurine is N-methylated | Tinospora cordifolia | S-adenosyl-L-homocysteine + (R)-N-methylnorcoclaurine | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
34 | 37 | 23fold purified enzyme preparation, pH 9.0 | Tinospora cordifolia |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | - |
narrow optimum | Tinospora cordifolia |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9.5 | - |
Tinospora cordifolia |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.062 | - |
S-adenosyl-L-methionine | substrate (S)-coclaurine | Tinospora cordifolia |