Literature summary for 2.1.1.14 extracted from

Wheatley, R.W.; Ng, K.K.S.; Kapoor, M.
Fungal cobalamin-independent methionine synthase Insights from the model organism, Neurospora crassa (2016), Arch. Biochem. Biophys., 590, 125-137 .

Crystallization (Commentary)

Crystallization (Comment)	Organism
structures of native MetE in complex with either Zn2+ or Cd2+, at resolution limits of 2.10 A and 1.88 A, respectively. The monomeric protein contains two domains, each containing a (betaalpha)8 barrel core, and a long alpha-helical segment spans the length of the protein, connecting the domains. Zn2+ bound in the C-terminal domain exhibits tetrahedral coordination with the side chains of His652, Cys654, Glu676 and Cys737	Neurospora crassa

Crystallization (Comment)

Organism

structures of native MetE in complex with either Zn2+ or Cd2+, at resolution limits of 2.10 A and 1.88 A, respectively. The monomeric protein contains two domains, each containing a (betaalpha)8 barrel core, and a long alpha-helical segment spans the length of the protein, connecting the domains. Zn2+ bound in the C-terminal domain exhibits tetrahedral coordination with the side chains of His652, Cys654, Glu676 and Cys737

Neurospora crassa

Metals/Ions

Metals/Ions	Comment	Organism	Structure
cadmium	Cd2+ may replace the Zn2+ ion in the active site	Neurospora crassa
Zinc	Zn2+ bound in the C-terminal domain exhibits tetrahedral coordination with the side chains of His652, Cys654, Glu676 and Cys737	Neurospora crassa

Organism

Organism	UniProt	Comment	Textmining
Neurospora crassa	Q8X1E4	-	-

Synonyms

Synonyms	Comment	Organism
Met-8	-	Neurospora crassa
MetE	-	Neurospora crassa

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
65	-	10 min, stable	Neurospora crassa

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Release 2023.1 (January 2023)