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Literature summary for 2.1.1.14 extracted from

  • Ubhi, D.K.; Robertus, J.D.
    The cobalamin-independent methionine synthase enzyme captured in a substrate-induced closed conformation (2015), J. Mol. Biol., 427, 901-909.
    View publication on PubMed

Application

Application Comment Organism
analysis development of a robust fluorescence-based activity assay suitable for high-throughput screening Candida albicans

Crystallization (Commentary)

Crystallization (Comment) Organism
binding of L-homocysteine or methionine results in conformational rearrangements at the amino acid binding pocket, moving the catalytic zinc into position to activate the thiol group. Active-site residues Asn126 and Tyr660 play key roles in catalysis Candida albicans

Protein Variants

Protein Variants Comment Organism
N126A 13% of wild-type activity Candida albicans
Y660A no residual activity Candida albicans
Y660F 93% of wild-type activity Candida albicans
Y660Q 14% of wild-type activity Candida albicans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
L-homocysteine 37°C, pH not specified in the publication Candida albicans
0.087
-
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate 37°C, pH not specified in the publication Candida albicans

Organism

Organism UniProt Comment Textmining
Candida albicans P82610
-
-
Candida albicans ATCC MYA-2876 P82610
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
Candida albicans tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
?
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
Candida albicans ATCC MYA-2876 tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
?

Synonyms

Synonyms Comment Organism
Met6
-
Candida albicans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.45
-
L-homocysteine 37°C, pH not specified in the publication Candida albicans
0.49
-
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate 37°C, pH not specified in the publication Candida albicans