Application | Comment | Organism |
---|---|---|
analysis | development of a robust fluorescence-based activity assay suitable for high-throughput screening | Candida albicans |
Crystallization (Comment) | Organism |
---|---|
binding of L-homocysteine or methionine results in conformational rearrangements at the amino acid binding pocket, moving the catalytic zinc into position to activate the thiol group. Active-site residues Asn126 and Tyr660 play key roles in catalysis | Candida albicans |
Protein Variants | Comment | Organism |
---|---|---|
N126A | 13% of wild-type activity | Candida albicans |
Y660A | no residual activity | Candida albicans |
Y660F | 93% of wild-type activity | Candida albicans |
Y660Q | 14% of wild-type activity | Candida albicans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
L-homocysteine | 37°C, pH not specified in the publication | Candida albicans | |
0.087 | - |
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate | 37°C, pH not specified in the publication | Candida albicans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida albicans | P82610 | - |
- |
Candida albicans ATCC MYA-2876 | P82610 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine | - |
Candida albicans | tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine | - |
? | |
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine | - |
Candida albicans ATCC MYA-2876 | tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Met6 | - |
Candida albicans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.45 | - |
L-homocysteine | 37°C, pH not specified in the publication | Candida albicans | |
0.49 | - |
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate | 37°C, pH not specified in the publication | Candida albicans |