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Literature summary for 2.1.1.137 extracted from

  • Huang, K.; Xu, Y.; Packianathan, C.; Gao, F.; Chen, C.; Zhang, J.; Shen, Q.; Rosen, B.P.; Zhao, F.J.
    Arsenic methylation by a novel ArsM As(III) S-adenosylmethionine methyltransferase that requires only two conserved cysteine residues (2018), Mol. Microbiol., 107, 265-276 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.137

Activating Compound

Activating Compound Comment Organism Structure
glutathione ArsM is able to methylate As(III) in the presence of Cys without glutathione. In contrast, ArsM is not able to methylate As(III) in the presence of tris(2-carboxyethyl)phosphine without GSH Bacillus sp. CX-1

Crystallization (Commentary)

Crystallization (Comment) Organism
homology modeling of structure. The two conserved cysteine residues Cys145 and Cys195 are in close propinquity and form an As(III) binding site. GSH forms the third ligand with As with the sulfur atom of GSH orienting toward the bound As(III) at approximately 2.4 A distance Bacillus sp. CX-1

Protein Variants

Protein Variants Comment Organism
C10S activtiy similar to wild-type Bacillus sp. CX-1
C10S/C11S/C193S/C268S mutant dispays activity Bacillus sp. CX-1
C11S activity similar to wild-type Bacillus sp. CX-1
C145S loss of catalytic activity Bacillus sp. CX-1
C193S activity similar to wild-type Bacillus sp. CX-1
C195S loss of catalytic activity Bacillus sp. CX-1
C268S activity similar to wild-type Bacillus sp. CX-1

Organism

Organism UniProt Comment Textmining
Bacillus sp. CX-1 A0A2P1M875
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + arsenite
-
 Bacillus sp. CX-1 S-adenosyl-L-homocysteine + methylarsonate
-
 ?
S-adenosyl-L-methionine + methylarsonite
-
 Bacillus sp. CX-1 S-adenosyl-L-homocysteine + dimethylarsinate
-
 ?

Subunits

Subunits Comment Organism
? x * 32000, calculated from sequence and SDS-PAGE, recombinant protein Bacillus sp. CX-1

Expression

Organism Comment Expression
Bacillus sp. CX-1 enzyme is constitutively expressed additional information

General Information

General Information Comment Organism
physiological function expression of ArsM confers resistance to an As(III)-hypersensitive strain of Escherichia coli Bacillus sp. CX-1