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Literature summary for 2.1.1.137 extracted from

  • Song, X.; Geng, Z.; Li, X.; Zhao, Q.; Hu, X.; Zhang, X.; Wang, Z.
    Functional and structural evaluation of cysteine residues in the human arsenic (+3 oxidation state) methyltransferase (hAS3MT) (2011), Biochimie, 93, 369-375.
    View publication on PubMed
Search for more literature for 2.1.1.137

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) pLysS cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
C271S the mutation does not affect the activity and structure of the enzyme Homo sapiens
C334S the mutation decreases the enzymatic turnover and changes the conformation of the enzyme Homo sapiens
C360S the mutation decreases the enzymatic turnover and changes the conformation of the enzyme Homo sapiens
C375S the mutation does not affect the activity and structure of the enzyme Homo sapiens
C72S the mutant is completely inactive Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0016
-
 arsenite mutant enzyme C334S, in PBS (25 mM, pH 7.0), at 37°C Homo sapiens
0.00188
-
 arsenite mutant enzyme C271S, in PBS (25 mM, pH 7.0), at 37°C Homo sapiens
0.00232
-
 arsenite mutant enzyme C360S, in PBS (25 mM, pH 7.0), at 37°C Homo sapiens
0.0032
-
 arsenite wild type enzyme, in PBS (25 mM, pH 7.0), at 37°C Homo sapiens
0.0185
-
 arsenite mutant enzyme C375S, in PBS (25 mM, pH 7.0), at 37°C Homo sapiens
0.0478
-
 S-adenosyl-L-methionine wild type enzyme, in PBS (25 mM, pH 7.0), at 37°C Homo sapiens
0.049
-
 S-adenosyl-L-methionine mutant enzyme C375S, in PBS (25 mM, pH 7.0), at 37°C Homo sapiens
0.0495
-
 S-adenosyl-L-methionine mutant enzyme C360S, in PBS (25 mM, pH 7.0), at 37°C Homo sapiens
0.0503
-
 S-adenosyl-L-methionine mutant enzyme C334S, in PBS (25 mM, pH 7.0), at 37°C Homo sapiens
0.0512
-
 S-adenosyl-L-methionine mutant enzyme C271S, in PBS (25 mM, pH 7.0), at 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + arsenite
-
 Homo sapiens S-adenosyl-L-homocysteine + methylarsonate
-
 ?

Synonyms

Synonyms Comment Organism
arsenic (+3 oxidation state) methyltransferase
-
 Homo sapiens
AS3MT
-
 Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 the wild type enzyme exhibits about 40% residual activity after 120 min at 45°C Homo sapiens