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Literature summary for 2.1.1.137 extracted from
- Arsenic (+3 oxidation state) methyltransferase and the methylation of arsenicals (2007), Exp. Biol. Med., 232, 3-13.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| GSH | addition of GSH stimulates the overall rate for As methylation, but inhibits formation of trimethylarsenite | Rattus norvegicus |  |
| GSH | addition of GSH stimulates the overall rate for As methylation, but inhibits formation of trimethylarsenite | Homo sapiens | |
| thioredoxin | activates formation of methyl arsenic, dimethyl arsenic, and trimethyl arsenic | Rattus norvegicus | |
| thioredoxin | dependent on, activates formation of methyl arsenic, dimethyl arsenic, and trimethyl arsenic | Homo sapiens | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of rat AS3MT in a simian virus 40 (SV40)-transformed human urothelial cell line confers the capacity to methylate inorganic arsenic on cells that otherwise do not express AS3MT and that have a null phenotype for iAs methylation | Rattus norvegicus |
| gene arsM, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, genotype-phenotype correlations for arsenic methylation and AS3MT | Gallus gallus |
| gene arsM, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, genotype-phenotype correlations for arsenic methylation and AS3MT | Mus musculus |
| gene arsM, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, genotype-phenotype correlations for arsenic methylation and AS3MT | Rhodopseudomonas palustris |
| gene arsM, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, genotype-phenotype correlations for arsenic methylation and AS3MT | Oncorhynchus mykiss |
| gene arsM, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, genotype-phenotype correlations for arsenic methylation and AS3MT | Strongylocentrotus purpuratus |
| gene arsM, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, genotype-phenotype correlations for arsenic methylation and AS3MT | Ciona intestinalis |
| gene arsM, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, genotype-phenotype correlations for arsenic methylation and AS3MT | Pan troglodytes |
| gene arsM, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, genotype-phenotype correlations for arsenic methylation and AS3MT | Rattus norvegicus |
| gene arsM, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, genotype-phenotype correlations for arsenic methylation and AS3MT | Bos taurus |
| gene arsM, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, genotype-phenotype correlations for arsenic methylation and AS3MT, recombinant expression | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| GSH | addition of GSH stimulates the overall rate for As methylation, but inhibits formation of trimethylarsenite | Homo sapiens | |
| GSH | addition of GSH stimulates the overall rate for As methylation, but inhibits formation of trimethylarsenite | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rattus norvegicus | 5829 | - |
| cytosol | - |
Homo sapiens | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
x * 42000, SDS-PAGE | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Gallus gallus | enzyme evolutionary analysis | ? | - |
? | |
| additional information | Mus musculus | enzyme evolutionary analysis | ? | - |
? | |
| additional information | Rhodopseudomonas palustris | enzyme evolutionary analysis | ? | - |
? | |
| additional information | Oncorhynchus mykiss | enzyme evolutionary analysis | ? | - |
? | |
| additional information | Strongylocentrotus purpuratus | enzyme evolutionary analysis | ? | - |
? | |
| additional information | Ciona intestinalis | enzyme evolutionary analysis | ? | - |
? | |
| additional information | Pan troglodytes | enzyme evolutionary analysis | ? | - |
? | |
| additional information | Rattus norvegicus | enzyme evolutionary analysis | ? | - |
? | |
| additional information | Homo sapiens | enzyme evolutionary analysis | ? | - |
? | |
| additional information | Bos taurus | enzyme evolutionary analysis | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Bos taurus | Q58DQ0 | gene arsM | - |
| Ciona intestinalis | - |
- |
- |
| Ciona intestinalis | - |
gene arsM | - |
| Gallus gallus | - |
- |
- |
| Gallus gallus | - |
gene arsM | - |
| Homo sapiens | Q9HBK9 | - |
- |
| Homo sapiens | Q9HBK9 | gene arsM | - |
| Mus musculus | - |
- |
- |
| Mus musculus | - |
gene arsM | - |
| no activity in Caenorhabditis elegans | - |
- |
- |
| no activity in Drosophila melanogaster | - |
- |
- |
| Oncorhynchus mykiss | - |
- |
- |
| Oncorhynchus mykiss | - |
gene arsM | - |
| Pan troglodytes | - |
- |
- |
| Pan troglodytes | - |
gene arsM | - |
| Rattus norvegicus | - |
- |
- |
| Rattus norvegicus | Q8VHT6 | adult male Fischer 344 rats, gene arsM | - |
| Rhodopseudomonas palustris | - |
gene arsM | - |
| Strongylocentrotus purpuratus | - |
gene arsM | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme several thousandfold using pH-dependent fractionation, chromatofocusing, and S-adenosylhomocysteine-affinity chromatography, the purification of AS3MT from liver cytosol is dependent on the presence of 1 mM dithiothreitol and 5 mM GSH | Rattus norvegicus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| S-adenosyl-L-methionine + arsenite = S-adenosyl-L-homocysteine + methylarsonate | reaction modelling, overview | Gallus gallus | |
| S-adenosyl-L-methionine + arsenite = S-adenosyl-L-homocysteine + methylarsonate | reaction modelling, overview | Mus musculus | |
| S-adenosyl-L-methionine + arsenite = S-adenosyl-L-homocysteine + methylarsonate | reaction modelling, overview | Rhodopseudomonas palustris | |
| S-adenosyl-L-methionine + arsenite = S-adenosyl-L-homocysteine + methylarsonate | reaction modelling, overview | Oncorhynchus mykiss | |
| S-adenosyl-L-methionine + arsenite = S-adenosyl-L-homocysteine + methylarsonate | reaction modelling, overview | Strongylocentrotus purpuratus | |
| S-adenosyl-L-methionine + arsenite = S-adenosyl-L-homocysteine + methylarsonate | reaction modelling, overview | Ciona intestinalis | |
| S-adenosyl-L-methionine + arsenite = S-adenosyl-L-homocysteine + methylarsonate | reaction modelling, overview | Pan troglodytes | |
| S-adenosyl-L-methionine + arsenite = S-adenosyl-L-homocysteine + methylarsonate | reaction modelling, overview | Rattus norvegicus | |
| S-adenosyl-L-methionine + arsenite = S-adenosyl-L-homocysteine + methylarsonate | reaction modelling, overview | Homo sapiens | |
| S-adenosyl-L-methionine + arsenite = S-adenosyl-L-homocysteine + methylarsonate | reaction modelling, overview | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| hepatocyte | primary | Homo sapiens | - |
| liver | - |
Rattus norvegicus | - |
| liver | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | enzyme evolutionary analysis | Gallus gallus | ? | - |
? | |
| additional information | enzyme evolutionary analysis | Mus musculus | ? | - |
? | |
| additional information | enzyme evolutionary analysis | Rhodopseudomonas palustris | ? | - |
? | |
| additional information | enzyme evolutionary analysis | Oncorhynchus mykiss | ? | - |
? | |
| additional information | enzyme evolutionary analysis | Strongylocentrotus purpuratus | ? | - |
? | |
| additional information | enzyme evolutionary analysis | Ciona intestinalis | ? | - |
? | |
| additional information | enzyme evolutionary analysis | Pan troglodytes | ? | - |
? | |
| additional information | enzyme evolutionary analysis | Rattus norvegicus | ? | - |
? | |
| additional information | enzyme evolutionary analysis | Homo sapiens | ? | - |
? | |
| additional information | enzyme evolutionary analysis | Bos taurus | ? | - |
? | |
| S-adenosyl-L-methionine + arsenite | - |
Gallus gallus | S-adenosyl-L-homocysteine + methylarsonate | - |
? | |
| S-adenosyl-L-methionine + arsenite | - |
Mus musculus | S-adenosyl-L-homocysteine + methylarsonate | - |
? | |
| S-adenosyl-L-methionine + arsenite | - |
Rattus norvegicus | S-adenosyl-L-homocysteine + methylarsonate | - |
? | |
| S-adenosyl-L-methionine + arsenite | - |
Bos taurus | S-adenosyl-L-homocysteine + methylarsonate | - |
? | |
| S-adenosyl-L-methionine + arsenite | - |
Oncorhynchus mykiss | S-adenosyl-L-homocysteine + methylarsonate | - |
? | |
| S-adenosyl-L-methionine + arsenite | - |
Ciona intestinalis | S-adenosyl-L-homocysteine + methylarsonate | - |
? | |
| S-adenosyl-L-methionine + arsenite | - |
Pan troglodytes | S-adenosyl-L-homocysteine + methylarsonate | - |
? | |
| S-adenosyl-L-methionine + arsenite | - |
Homo sapiens | S-adenosyl-L-homocysteine + methylarsonate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 42000, SDS-PAGE | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| arsenic (+3 oxidation state) methyltransferase | - |
Gallus gallus |
| arsenic (+3 oxidation state) methyltransferase | - |
Mus musculus |
| arsenic (+3 oxidation state) methyltransferase | - |
Rattus norvegicus |
| arsenic (+3 oxidation state) methyltransferase | - |
Bos taurus |
| arsenic (+3 oxidation state) methyltransferase | - |
Rhodopseudomonas palustris |
| arsenic (+3 oxidation state) methyltransferase | - |
Oncorhynchus mykiss |
| arsenic (+3 oxidation state) methyltransferase | - |
Strongylocentrotus purpuratus |
| arsenic (+3 oxidation state) methyltransferase | - |
Ciona intestinalis |
| arsenic (+3 oxidation state) methyltransferase | - |
Pan troglodytes |
| arsenic (+3 oxidation state) methyltransferase | - |
Homo sapiens |
| ArsM protein | - |
Gallus gallus |
| ArsM protein | - |
Mus musculus |
| ArsM protein | - |
Rhodopseudomonas palustris |
| ArsM protein | - |
Oncorhynchus mykiss |
| ArsM protein | - |
Strongylocentrotus purpuratus |
| ArsM protein | - |
Ciona intestinalis |
| ArsM protein | - |
Pan troglodytes |
| ArsM protein | - |
Rattus norvegicus |
| ArsM protein | - |
Homo sapiens |
| ArsM protein | - |
Bos taurus |
| AS3MT | - |
Gallus gallus |
| AS3MT | - |
Mus musculus |
| AS3MT | - |
Rattus norvegicus |
| AS3MT | - |
Bos taurus |
| AS3MT | - |
Rhodopseudomonas palustris |
| AS3MT | - |
Oncorhynchus mykiss |
| AS3MT | - |
Strongylocentrotus purpuratus |
| AS3MT | - |
Ciona intestinalis |
| AS3MT | - |
Pan troglodytes |
| AS3MT | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Rattus norvegicus |
| 7 | 7.4 | assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| S-adenosyl-L-methionine | - |
Gallus gallus | |
| S-adenosyl-L-methionine | - |
Mus musculus | |
| S-adenosyl-L-methionine | - |
Rhodopseudomonas palustris | |
| S-adenosyl-L-methionine | - |
Oncorhynchus mykiss | |
| S-adenosyl-L-methionine | - |
Strongylocentrotus purpuratus | |
| S-adenosyl-L-methionine | - |
Ciona intestinalis | |
| S-adenosyl-L-methionine | - |
Pan troglodytes | |
| S-adenosyl-L-methionine | - |
Rattus norvegicus | |
| S-adenosyl-L-methionine | - |
Homo sapiens | |
| S-adenosyl-L-methionine | - |
Bos taurus | |
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