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Literature summary for 2.1.1.132 extracted from

  • Blanche, F.; Famechon, A.; Thibaut, D.; Debussche, L.; Cameron, B.; Crouzet, J.
    Biosynthesis of vitamin B12 in Pseudomonas denitrificans: the biosynthetic sequence from precorrin-6y to precorrin-8x is catalyzed by the cobL gene product (1992), J. Bacteriol., 174, 1050-1052.
    View publication on PubMedView publication on EuropePMC
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0022
-
 Precorrin-6Y
-
 Pseudomonas denitrificans (nom. rej.)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42900
-
 x * 42900, calculation from nucleotide sequence Pseudomonas denitrificans (nom. rej.)
43000
-
 x * 43000, SDS-PAGE Pseudomonas denitrificans (nom. rej.)
320000
-
 HPLC gel filtration Pseudomonas denitrificans (nom. rej.)

Organism

Organism UniProt Comment Textmining
Pseudomonas denitrificans (nom. rej.)
-
 harboring plasmid pXL253
-
Pseudomonas denitrificans (nom. rej.) SC510 Rif
-
 harboring plasmid pXL253
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas denitrificans (nom. rej.)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0057
-
-
 Pseudomonas denitrificans (nom. rej.)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Adenosyl-L-methionine + precorrin-6Y
-
 Pseudomonas denitrificans (nom. rej.) Adenosyl-L-homocysteine + precorrin-8X + CO2
-
 ?
Adenosyl-L-methionine + precorrin-6Y
-
 Pseudomonas denitrificans (nom. rej.) SC510 Rif Adenosyl-L-homocysteine + precorrin-8X + CO2
-
 ?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE Pseudomonas denitrificans (nom. rej.)
? x * 42900, calculation from nucleotide sequence Pseudomonas denitrificans (nom. rej.)