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Literature summary for 2.1.1.13 extracted from
- N5-Methytetrahydrofolate-homocysteine (vitamin B12) methyltransferase (Escherichia coli B) (1971), Methods Enzymol., 17B, 379-388.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0016 | - |
S-adenosyl-L-methionine | - |
Escherichia coli |  |
| 0.016 | - |
L-homocysteine | - |
Escherichia coli | |
| 0.06 | - |
5-methyltetrahydrofolate | - |
Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 140000 | - |
sucrose density gradient centrifugation | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
B | - |
| Escherichia coli B / ATCC 11303 | - |
B | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| B | Escherichia coli |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 5-methyltetrahydrofolate + L-homocysteine = tetrahydrofolate + L-methionine | mechanism | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
assay method | Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 15-20 mg/ml protein, stable for at least 1 month | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-methyltetrahydrofolate + L-homocysteine | - |
Escherichia coli | tetrahydrofolate + L-methionine | - |
? | |
| 5-methyltetrahydrofolate + L-homocysteine | - |
Escherichia coli B / ATCC 11303 | tetrahydrofolate + L-methionine | - |
? | |
| N5-methyltetrahydrofolate + L-homocysteine | - |
Escherichia coli | tetrahydrofolate + L-methionine | - |
? | |
| N5-methyltetrahydrofolate + L-homocysteine | - |
Escherichia coli B / ATCC 11303 | tetrahydrofolate + L-methionine | - |
? | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| vitamin B12 | enzyme contains a derivative of vitamin B12 as prosthetic group | Escherichia coli | |
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Release 2023.1 (January 2023)
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