Application | Comment | Organism |
---|---|---|
synthesis | production of the economically important analgesic morphine and the antimicrobial agent berberine | Eschscholzia californica |
synthesis | production of the economically important analgesic morphine and the antimicrobial agent berberine | Coptis japonica |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Eschscholzia californica |
expressed in Eschscholzia californica | Coptis japonica |
full-length cDNA of Coptis japonica 6OMT is cloned into the binary vector pBITXEl2 for introduction into Agrobacterium tumefaciens, for infection Eschscholzia californica seedlings are co-cultured with Agrobacterium tumefaciens | Coptis japonica |
into the pET-21d vector for expression in Escherichia coli BL21DE3 cells | Coptis japonica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, SDS-PAGE, recombinant Ec4OMT | Eschscholzia californica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-homocysteine + (S)-reticuline | Coptis japonica | - |
S-adenosyl-L-methionine + 3'-hydroxy-N-methyl-(S)-coclaurine | - |
? | |
S-adenosyl-L-methionine + (S)-norcoclaurine | Coptis japonica | - |
S-adenosyl-L-homocysteine + (S)-coclaurine | - |
? | |
S-adenosyl-L-methionine + (S)-norcoclaurine | Coptis japonica | putative rate-limiting step enzymes in benzylisoquinoline alkaloid biosynthesis | S-adenosyl-L-homocysteine + (S)-coclaurine | - |
? | |
S-adenosyl-L-methionine + (S)-norlaudanosoline | Eschscholzia californica | putative rate-limiting step enzymes in benzylisoquinoline alkaloid biosynthesis | S-adenosyl-L-homocysteine + 6-O-methylnorlaudanosoline | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Coptis japonica | Q9LEL6 | - |
- |
Eschscholzia californica | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Coptis japonica | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-homocysteine + (S)-reticuline | - |
Coptis japonica | S-adenosyl-L-methionine + 3'-hydroxy-N-methyl-(S)-coclaurine | - |
? | |
S-adenosyl-L-methionine + (S)-norcoclaurine | - |
Eschscholzia californica | S-adenosyl-L-homocysteine + (S)-coclaurine | - |
? | |
S-adenosyl-L-methionine + (S)-norcoclaurine | - |
Coptis japonica | S-adenosyl-L-homocysteine + (S)-coclaurine | - |
? | |
S-adenosyl-L-methionine + (S)-norcoclaurine | putative rate-limiting step enzymes in benzylisoquinoline alkaloid biosynthesis | Coptis japonica | S-adenosyl-L-homocysteine + (S)-coclaurine | - |
? | |
S-adenosyl-L-methionine + (S)-norlaudanosoline | putative rate-limiting step enzymes in benzylisoquinoline alkaloid biosynthesis | Eschscholzia californica | S-adenosyl-L-homocysteine + 6-O-methylnorlaudanosoline | - |
? | |
S-adenosyl-L-methionine + laudanosoline | low activity | Eschscholzia californica | ? | - |
? | |
S-adenosyl-L-methionine + laudanosoline | low activity | Coptis japonica | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE, recombinant Ec4OMT | Eschscholzia californica |
Synonyms | Comment | Organism |
---|---|---|
3-hydroxy-N-methylcoclaurine 4-O-methyltransferase | Ec4OMT shows clear 6-O-methylation activity for norcoclaurine and produced coclaurine | Eschscholzia californica |
6OMT | - |
Coptis japonica |
Ec4OMT | - |
Eschscholzia californica |
Norcoclaurine 6-O-methyltransferase | - |
Eschscholzia californica |
Norcoclaurine 6-O-methyltransferase | - |
Coptis japonica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
OMT enzyme assay | Coptis japonica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
OMT enzyme assay | Coptis japonica |