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Literature summary for 2.1.1.128 extracted from

  • Inui, T.; Tamura, K.; Fujii, N.; Morishige, T.; Sato, F.
    Overexpression of Coptis japonica norcoclaurine 6-O-methyltransferase overcomes the rate-limiting step in Benzylisoquinoline alkaloid biosynthesis in cultured Eschscholzia californica (2007), Plant Cell Physiol., 48, 252-262.
    View publication on PubMed

Application

Application Comment Organism
synthesis production of the economically important analgesic morphine and the antimicrobial agent berberine Eschscholzia californica
synthesis production of the economically important analgesic morphine and the antimicrobial agent berberine Coptis japonica

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Eschscholzia californica
expressed in Eschscholzia californica Coptis japonica
full-length cDNA of Coptis japonica 6OMT is cloned into the binary vector pBITXEl2 for introduction into Agrobacterium tumefaciens, for infection Eschscholzia californica seedlings are co-cultured with Agrobacterium tumefaciens Coptis japonica
into the pET-21d vector for expression in Escherichia coli BL21DE3 cells Coptis japonica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
x * 40000, SDS-PAGE, recombinant Ec4’OMT Eschscholzia californica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-homocysteine + (S)-reticuline Coptis japonica
-
S-adenosyl-L-methionine + 3'-hydroxy-N-methyl-(S)-coclaurine
-
?
S-adenosyl-L-methionine + (S)-norcoclaurine Coptis japonica
-
S-adenosyl-L-homocysteine + (S)-coclaurine
-
?
S-adenosyl-L-methionine + (S)-norcoclaurine Coptis japonica putative rate-limiting step enzymes in benzylisoquinoline alkaloid biosynthesis S-adenosyl-L-homocysteine + (S)-coclaurine
-
?
S-adenosyl-L-methionine + (S)-norlaudanosoline Eschscholzia californica putative rate-limiting step enzymes in benzylisoquinoline alkaloid biosynthesis S-adenosyl-L-homocysteine + 6-O-methylnorlaudanosoline
-
?

Organism

Organism UniProt Comment Textmining
Coptis japonica Q9LEL6
-
-
Eschscholzia californica
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
Coptis japonica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-homocysteine + (S)-reticuline
-
Coptis japonica S-adenosyl-L-methionine + 3'-hydroxy-N-methyl-(S)-coclaurine
-
?
S-adenosyl-L-methionine + (S)-norcoclaurine
-
Eschscholzia californica S-adenosyl-L-homocysteine + (S)-coclaurine
-
?
S-adenosyl-L-methionine + (S)-norcoclaurine
-
Coptis japonica S-adenosyl-L-homocysteine + (S)-coclaurine
-
?
S-adenosyl-L-methionine + (S)-norcoclaurine putative rate-limiting step enzymes in benzylisoquinoline alkaloid biosynthesis Coptis japonica S-adenosyl-L-homocysteine + (S)-coclaurine
-
?
S-adenosyl-L-methionine + (S)-norlaudanosoline putative rate-limiting step enzymes in benzylisoquinoline alkaloid biosynthesis Eschscholzia californica S-adenosyl-L-homocysteine + 6-O-methylnorlaudanosoline
-
?
S-adenosyl-L-methionine + laudanosoline low activity Eschscholzia californica ?
-
?
S-adenosyl-L-methionine + laudanosoline low activity Coptis japonica ?
-
?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE, recombinant Ec4’OMT Eschscholzia californica

Synonyms

Synonyms Comment Organism
3’-hydroxy-N-methylcoclaurine 4’-O-methyltransferase Ec4’OMT shows clear 6-O-methylation activity for norcoclaurine and produced coclaurine Eschscholzia californica
6OMT
-
Coptis japonica
Ec4’OMT
-
Eschscholzia californica
Norcoclaurine 6-O-methyltransferase
-
Eschscholzia californica
Norcoclaurine 6-O-methyltransferase
-
Coptis japonica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
OMT enzyme assay Coptis japonica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
OMT enzyme assay Coptis japonica