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Literature summary for 2.1.1.128 extracted from

  • Samanani, N.; Alcantara, J.; Bourgault, R.; Zulak, K.G.; Facchini, P.J.
    The role of phloem sieve elements and laticifers in the biosynthesis and accumulation of alkaloids in opium poppy (2006), Plant J., 47, 547-563.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed as His-tag fusion protein in Escherichia coli ER2566pLys S Papaver somniferum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm immunogold labelling studies show the strict association with electron dense regions of the peripheral cytoplasm of sieve elements Papaver somniferum 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38500
-
x * 38500, immunoblotting Papaver somniferum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + (S)-norcoclaurine Papaver somniferum putative rate-limiting step enzymes in benzylisoquinoline alkaloid biosynthesis S-adenosyl-L-homocysteine + (S)-coclaurine
-
?

Organism

Organism UniProt Comment Textmining
Papaver somniferum
-
cv. Louisiana and Marianne
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein using His-tag Papaver somniferum

Source Tissue

Source Tissue Comment Organism Textmining
flower low enzyme level Papaver somniferum
-
leaf low enzyme level Papaver somniferum
-
additional information gene transcripts detected in all organs with highest levels in root and stem and lowest in leaf Papaver somniferum
-
phloem sieve elements of the phloem adjacent or proximal to laticifers Papaver somniferum
-
root
-
Papaver somniferum
-
seed constant enzyme level over 16 days of germination Papaver somniferum
-
stem
-
Papaver somniferum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + (S)-norcoclaurine putative rate-limiting step enzymes in benzylisoquinoline alkaloid biosynthesis Papaver somniferum S-adenosyl-L-homocysteine + (S)-coclaurine
-
?

Subunits

Subunits Comment Organism
? x * 38500, immunoblotting Papaver somniferum

Synonyms

Synonyms Comment Organism
(S)-norcoclaurine 6-O-methyltransferase
-
Papaver somniferum
6OMT
-
Papaver somniferum