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Literature summary for 2.1.1.122 extracted from
- Partial purification and properties of S-adenosyl-L-methionine:(S)-tetrahydroprotoberberine-cis-N-methyltransferase from suspension-cultured cells of Eschscholtzia and Corydalis (1990), Phytochemistry, 29, 3727-3733.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| S-adenosyl-L-homocysteine | - |
Eschscholzia californica |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0017 | - |
S-adenosyl-L-methionine | - |
Corydalis vaginans | |
| 0.0031 | - |
(S)-Stylopine | - |
Corydalis vaginans | |
| 0.004 | - |
(S)-Stylopine | - |
Eschscholzia californica | |
| 0.0064 | - |
(S)-canadine | - |
Eschscholzia californica | |
| 0.007 | - |
(S)-canadine | - |
Corydalis vaginans | |
| 0.012 | - |
S-adenosyl-L-methionine | - |
Eschscholzia californica | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| additional information | the enzyme is not compartmentalized | Eschscholzia californica | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 72000 | - |
gel filtration | Corydalis vaginans |
| 78000 | - |
gel filtration | Eschscholzia californica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Corydalis vaginans | - |
- |
- |
| Eschscholzia californica | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Eschscholzia californica |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell suspension culture | - |
Eschscholzia californica | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Eschscholzia californica |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 20% glycerol, half-life: 5 days | Eschscholzia californica |
| 4°C, 20% glycerol, half-life: 10 days | Eschscholzia californica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-Adenosyl-L-methionine + (S)-7,8,13,14-tetrahydroprotoberberine | - |
Eschscholzia californica | S-Adenosyl-L-homocysteine + cis-N-methyl-(S)-7,8,13,14-tetrahydroprotoberberine | - |
? | |
| S-Adenosyl-L-methionine + (S)-7,8,13,14-tetrahydroprotoberberine | - |
Corydalis vaginans | S-Adenosyl-L-homocysteine + cis-N-methyl-(S)-7,8,13,14-tetrahydroprotoberberine | - |
? | |
| S-adenosyl-L-methionine + (S)-canadine | - |
Eschscholzia californica | ? | - |
? | |
| S-adenosyl-L-methionine + (S)-canadine | - |
Corydalis vaginans | ? | - |
? | |
| S-Adenosyl-L-methionine + (S)-stylopine | - |
Eschscholzia californica | ? | - |
? | |
| S-Adenosyl-L-methionine + (S)-stylopine | - |
Corydalis vaginans | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 40 | - |
Eschscholzia californica |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
half-life: 5 days | Eschscholzia californica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.9 | - |
- |
Eschscholzia californica |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | 9.1 | about 50% of maximal activity at pH 6.6 and 9.1 | Eschscholzia californica |
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