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Literature summary for 2.1.1.116 extracted from

  • Samanani, N.; Alcantara, J.; Bourgault, R.; Zulak, K.G.; Facchini, P.J.
    The role of phloem sieve elements and laticifers in the biosynthesis and accumulation of alkaloids in opium poppy (2006), Plant J., 47, 547-563.
    View publication on PubMed

Application

Application Comment Organism
additional information localization of seven biosynthetic enzymes to the sieve elements, unique, cell type-specific biosynthesis of benzylisoquinoline alkaloids in the opium poppy Papaver somniferum

Cloned(Commentary)

Cloned (Comment) Organism
into pRSETB and introduced into Escherichia coli strain ER2566 pLys S Papaver somniferum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Papaver somniferum 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39400
-
immunoblot analysis Papaver somniferum

Organism

Organism UniProt Comment Textmining
Papaver somniferum
-
cultivars Louisiana and Marianne
-

Purification (Commentary)

Purification (Comment) Organism
by Ni2+ affinity chromatography Papaver somniferum

Source Tissue

Source Tissue Comment Organism Textmining
flower bud higher levels of 4'OMT mRNA in cultivar Marianne, compared with cultivar Louisiana Papaver somniferum
-
hypocotyl
-
Papaver somniferum
-
leaf lowest gene transcripts Papaver somniferum
-
phloem restricted to sieve elements adjacent or proximal to laticifers Papaver somniferum
-
root highest gene transcripts Papaver somniferum
-
seedling abundance of the enzyme increases rapidly between 1 and 3 days after seed germination Papaver somniferum
-
stem highest gene transcripts and levels of protein Papaver somniferum
-

Synonyms

Synonyms Comment Organism
(S)-3'-hydroxy-N-methylcoclaurine-4'-O-methyltransferase
-
Papaver somniferum
4'OMT
-
Papaver somniferum