Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.113 extracted from

  • Yu, Y.J.; Yang, M.T.
    A novel restriction-modification system from Xanthomonas campestris pv. vesicatoria encodes a m4C-methyltransferase and a nonfunctional restriction endonuclease (2007), FEMS Microbiol. Lett., 272, 83-90.
    View publication on PubMed
Search for more literature for 2.1.1.113

Cloned(Commentary)

Cloned (Comment) Organism
obtained from genomic library, amplified in Escherichia coli XL1-Blue, strain ER2566 for expression of the M.XveII carrying a C-terminal His6-tag Xanthomonas campestris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18300
-
 truncated protein predicted due to translation termination codon in the open reading frame Xanthomonas campestris
337000
-
 299-amino acid protein, predicted by sequence Xanthomonas campestris

Organism

Organism UniProt Comment Textmining
Xanthomonas campestris Q3BWM2 pv. vesicatoria, strains Xcv7-1, Xv2 and Xv64 and ATCC 35937 strain
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration, recombinant protein for in vitro methyltransferase activity assay Xanthomonas campestris

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Xanthomonas campestris
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 genetic organization of novel restriction-modification (R-M) system, tail-to-tail gene orientation with 12 base pairs overlapping, protection and activity assays, classified as member of beta-class of m4C-methyltransferases, predicted amino acid sequence reveals 41.9% identity with SmaI, no SmaI-like restriction activity in cell extracts of the Xcv7-1 strain Xanthomonas campestris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + [DNA]-cytosine methylation of second cytosine of 5'-CCCGGG-3' recognition sequence Xanthomonas campestris S-adenosyl-L-homocysteine + [DNA]-N4-methylcytosine
-
 ?

Synonyms

Synonyms Comment Organism
M.XveII protein
-
 Xanthomonas campestris