Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no apparent substrate inhibition at concentrations 100fold higher than the Km for DNA and 20fold higher than Km for S-adenosyl-L-methionine | Bacillus amyloliquefaciens | |
S-adenosyl-L-homocysteine | competitive with regard to S-adenosylmethionine and non-competitive with respect to unmethylated 20-mer duplex | Bacillus amyloliquefaciens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus amyloliquefaciens | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + DNA cytosine = S-adenosyl-L-homocysteine + DNA N4-methylcytosine | the product inhibition pattern consistent with a steady-state random bi-bi mechanism in which the dominant order of substrate binding and product release: S-adenosyl-L-methionine, methylated DNA, DNAMe, S-adenosyl-L-homocysteine | Bacillus amyloliquefaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + [DNA]-cytosine | 20-mer oligodeoxynucleotide duplex containing the palindromic recognition site GGATCC. The enzyme catalyzes methyl group transfer to the internal cytosine residue in the palindromic recognition site GGATCC. The enzyme transfers the methyl group to the exocyclic NH2-group without formation of a transient covalent bond | Bacillus amyloliquefaciens | S-adenosyl-L-homocysteine + [DNA]-N4-methylcytosine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BamHI [cytosine-N4] MTase | - |
Bacillus amyloliquefaciens |