BRENDA - Enzyme Database show
show all sequences of 2.1.1.11

Structural insights into the catalytic mechanism of Synechocystis magnesium protoporphyrin IX O-methyltransferase (ChlM)

Chen, X.; Wang, X.; Feng, J.; Chen, Y.; Fang, Y.; Zhao, S.; Zhao, A.; Zhang, M.; Liu, L.; J. Biol. Chem. 289, 25690-25698 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Synechocystis sp.
Crystallization (Commentary)
Crystallization
Organism
in complex with S-adenosylmethionine and S-adenosylhomocysteine at resolutions of 1.6 and 1.7 A, respectively. Conformational changes of the two arm regions may modulate binding and release of substrates/products to and from the active site. Residues Tyr28 and His 139 play essential roles for methyl transfer reaction but are not indispensable for cofactor/substrate binding
Synechocystis sp.
Engineering
Amino acid exchange
Commentary
Organism
H139A
loss of more than 80% of methyltransferase activity, mutant has comparable Kd values for Mg-phosphate as the wild type protein
Synechocystis sp.
H139N
loss of more than 80% of methyltransferase activity, mutant has comparable Kd values for Mg-phosphate as the wild type protein
Synechocystis sp.
H139Q
loss of more than 80% of methyltransferase activity, mutant has comparable Kd values for Mg-phosphate as the wild type protein
Synechocystis sp.
Y28A
loss of more than 80% of methyltransferase activity, mutant has comparable Kd values for Mg-phosphate as the wild type protein
Synechocystis sp.
Y28F
mutation leads to inclusion body formation during bacterial expression
Synechocystis sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Synechocystis sp.
Q55467
-
-
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Synechocystis sp.
Crystallization (Commentary) (protein specific)
Crystallization
Organism
in complex with S-adenosylmethionine and S-adenosylhomocysteine at resolutions of 1.6 and 1.7 A, respectively. Conformational changes of the two arm regions may modulate binding and release of substrates/products to and from the active site. Residues Tyr28 and His 139 play essential roles for methyl transfer reaction but are not indispensable for cofactor/substrate binding
Synechocystis sp.
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
H139A
loss of more than 80% of methyltransferase activity, mutant has comparable Kd values for Mg-phosphate as the wild type protein
Synechocystis sp.
H139N
loss of more than 80% of methyltransferase activity, mutant has comparable Kd values for Mg-phosphate as the wild type protein
Synechocystis sp.
H139Q
loss of more than 80% of methyltransferase activity, mutant has comparable Kd values for Mg-phosphate as the wild type protein
Synechocystis sp.
Y28A
loss of more than 80% of methyltransferase activity, mutant has comparable Kd values for Mg-phosphate as the wild type protein
Synechocystis sp.
Y28F
mutation leads to inclusion body formation during bacterial expression
Synechocystis sp.
Other publictions for EC 2.1.1.11
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
734272
Chen
Structural insights into the c ...
Synechocystis sp.
J. Biol. Chem.
289
25690-25698
2014
-
-
1
1
5
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
734560
Tomiyama
Mg-chelatase I subunit 1 and M ...
Arabidopsis thaliana
J. Plant Res.
127
553-563
2014
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
720712
Meinecke
Chlorophyll-deficient mutants ...
Chlamydomonas reinhardtii
Plant Mol. Biol.
72
643-658
2010
-
-
1
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
695554
McLean
An enzyme-coupled continuous s ...
Synechocystis sp. PCC 6803
Anal. Biochem.
394
223-228
2009
-
1
1
-
-
-
-
-
-
1
-
1
-
1
-
-
1
-
-
-
-
-
2
-
1
-
-
-
2
-
-
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
2
-
1
-
-
-
2
-
-
-
1
-
-
1
-
-
700486
Van Wilder
C1 metabolism and chlorophyll ...
Arabidopsis thaliana, Pisum sativum
New Phytol.
182
137-145
2009
-
-
1
-
-
-
-
-
-
2
2
2
-
4
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
2
2
2
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
1
2
2
1
-
-
687761
Johnson
Characterization of three homo ...
Chlorobaculum tepidum
J. Biol. Chem.
283
27776-27784
2008
-
-
1
-
-
-
-
2
-
-
-
-
-
4
-
-
1
-
-
1
1
-
2
1
1
-
-
2
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
1
1
-
2
1
1
-
-
2
1
-
-
-
-
-
-
-
-
-
674841
Pontier
Knock-out of the magnesium pro ...
Arabidopsis thaliana
J. Biol. Chem.
282
2297-2304
2007
-
-
1
-
-
-
-
-
3
-
2
1
-
2
-
1
1
-
-
2
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
2
1
-
-
1
1
-
2
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684982
Sawicki
S-adenosyl-L-methionine:magnes ...
Rhodobacter capsulatus
Biochem. J.
406
469-478
2007
1
-
1
-
-
1
5
4
-
-
1
1
-
1
-
-
1
-
-
1
1
-
5
1
1
-
-
3
1
1
-
-
4
-
-
1
-
1
-
-
-
1
-
5
4
4
-
-
1
1
-
-
-
1
-
1
1
-
5
1
1
-
-
3
1
1
-
-
-
-
-
-
-
-
660178
Alawady
Tobacco Mg protoporphyrin IX m ...
Nicotiana tabacum
Plant J.
41
282-290
2005
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
660202
Alawady
Cloning and expression of the ...
Nicotiana tabacum
Plant Mol. Biol.
57
679-691
2005
-
-
1
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
661705
Shepherd
Kinetic basis for linking the ...
Synechocystis sp.
FEBS J.
272
4532-4539
2005
1
-
1
-
-
-
-
2
-
-
-
-
-
2
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657843
Shepherd
Transient kinetics of the reac ...
Synechocystis sp. PCC 6803
Biochem. J.
382
1009-1013
2004
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485113
Shepherd
Purification and kinetic chara ...
Synechocystis sp., Synechocystis sp. PCC 6803
Biochem. J.
371
351-360
2003
-
-
-
-
-
-
1
2
-
-
-
-
-
3
-
-
2
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
2
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485108
Block
The plant S-adenosyl-L-methion ...
Arabidopsis thaliana, Spinacia oleracea
Eur. J. Biochem.
269
240-248
2002
-
-
1
-
-
-
-
-
3
-
-
-
-
2
-
1
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485111
Averina
Native state, energetic intera ...
Hordeum vulgare
Indian J. Exp. Biol.
40
192-201
2002
-
-
-
-
-
-
-
-
2
-
-
-
-
3
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485112
Vothknecht
-
Sinefungin inhibits chlorophyl ...
Hordeum vulgare
Plant Physiol. Biochem.
33
759-763
1995
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485109
Gibson
The bacteriochlorophyll biosyn ...
Rhodobacter sphaeroides
FEBS Lett.
352
127-130
1994
-
-
1
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485110
Bollivar
Heterologous expression of the ...
Rhodobacter capsulatus
J. Bacteriol.
176
5290-5296
1994
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485102
Yee
Confirmation of a ping-pong me ...
Triticum aestivum
Biochem. Biophys. Res. Commun.
162
483-490
1989
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485104
Hinchigeri
-
The reaction mechanism of S-ad ...
Euglena gracilis
Photosynthetica
18
168-178
1984
-
-
-
-
-
-
3
-
-
-
-
-
-
1
-
-
1
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485103
Hinchigeri
-
The purification and reaction ...
Rhodobacter sphaeroides
Photosynthetica
16
554-560
1982
-
-
-
-
-
-
1
-
1
-
-
-
-
1
-
-
1
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485106
Hinchigeri
-
Purification of S-adenosyl-L-m ...
Hordeum vulgare
Photosynthetica
15
351-359
1981
1
-
-
-
-
-
3
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
2
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485107
Ellsworth
-
Biosynthesis and inhibition of ...
Triticum aestivum
Photosynthetica
10
291-301
1976
-
-
-
-
-
-
5
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
1
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485105
Ellsworth
Activity and properties of (-) ...
Triticum aestivum
Biochim. Biophys. Acta
268
327-333
1972
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
485101
Gibson
Studies on the biosynthesis of ...
Rhodobacter sphaeroides
Biochem. J.
88
325-334
1963
-
-
-
-
-
-
15
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
6
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
15
-
-
1
-
-
-
-
-
-
-
-
1
-
-
6
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-