Application | Comment | Organism |
---|---|---|
analysis | a continuous enzyme-coupled spectrophotometric assay for ChlM from Synechocystis sp. PCC 6803 is reported | Synechocystis sp. PCC 6803 |
Cloned (Comment) | Organism |
---|---|
into the vector pET9a for expression in Escherichia coli BL21DE3 cells | Synechocystis sp. PCC 6803 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Magnesium | - |
Synechocystis sp. PCC 6803 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + magnesium protoporphyrin IX | Synechocystis sp. PCC 6803 | - |
S-adenosyl-L-homocysteine + magnesium protoporphyrin IX 13-methyl ester | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechocystis sp. PCC 6803 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using a chelating Sepharose Fast Flow resin charged with 50 mM NiSO4 | Synechocystis sp. PCC 6803 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + magnesium deuteroporphyrin IX | activity assay | Synechocystis sp. PCC 6803 | S-adenosyl-L-homocysteine + magnesium deuteroporphyrin IX 13-methyl ester | - |
? | |
S-adenosyl-L-methionine + magnesium protoporphyrin IX | - |
Synechocystis sp. PCC 6803 | S-adenosyl-L-homocysteine + magnesium protoporphyrin IX 13-methyl ester | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ChlM | - |
Synechocystis sp. PCC 6803 |
magnesium protoporphyrin IX methyltransferase | - |
Synechocystis sp. PCC 6803 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
activity assay | Synechocystis sp. PCC 6803 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
continuous spectrophotometric assay | Synechocystis sp. PCC 6803 |
7.5 | - |
HPLC-based assay | Synechocystis sp. PCC 6803 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Synechocystis sp. PCC 6803 |
Organism | Comment | Expression |
---|---|---|
Synechocystis sp. PCC 6803 | the H subunit of Mg chelatase, ChlH, stimulates ChlM activity | up |