Cloned (Comment) | Organism |
---|---|
in Escherichia coli CBK103, amino acids 202-457 responsible for EC 2.1.1.107 activity | Arabidopsis thaliana |
General Stability | Organism |
---|---|
complete loss of activity when treated with Triton X-100 | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Arabidopsis thaliana | 9507 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39900 | - |
calculated from DNA sequence | Arabidopsis thaliana |
41000 | - |
SDS-PAGE | Arabidopsis thaliana |
67000 | - |
SDS-PAGE, uncleaved fusion protein | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + uroporphyrinogen III | Arabidopsis thaliana | involved in biosynthesis of siroheme | 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ | product is precorrin-2 | ? | |
2 S-adenosyl-L-methionine + uroporphyrinogen III | Arabidopsis thaliana | no synthesis of cobalamin | 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ | product is precorrin-2 | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q96532 | genomic DNA, cDNA has accession number L47479 | - |
Purification (Comment) | Organism |
---|---|
from recombinant E. coli | Arabidopsis thaliana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0091 | - |
purified enzyme | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + uroporphyrinogen III | - |
Arabidopsis thaliana | 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ | product is precorrin-2 | ? | |
2 S-adenosyl-L-methionine + uroporphyrinogen III | involved in biosynthesis of siroheme | Arabidopsis thaliana | 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ | product is precorrin-2 | ? | |
2 S-adenosyl-L-methionine + uroporphyrinogen III | no synthesis of cobalamin | Arabidopsis thaliana | 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ | product is precorrin-2 | ? | |
additional information | no formation of sirohydrochlorin in presence of NAD+ or NADP+ | Arabidopsis thaliana | ? | - |
? |