Cloned (Comment) | Organism |
---|---|
50 fold increase of enzyme activity when overexpressed | Pseudomonas denitrificans (nom. rej.) |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by cobalamine or intermediates of synthesis | Pseudomonas denitrificans (nom. rej.) | |
S-adenosyl-L-homocysteine | competitive | Pseudomonas denitrificans (nom. rej.) | |
uroporphyrinogen III | substrate inhibition above 0.002 mM | Pseudomonas denitrificans (nom. rej.) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
uroporphyrinogen III | - |
Pseudomonas denitrificans (nom. rej.) | |
0.0034 | - |
pentacarboxyporphyrinogen III | - |
Pseudomonas denitrificans (nom. rej.) | |
0.0042 | - |
heptacarboxyporphyrinogen III | - |
Pseudomonas denitrificans (nom. rej.) | |
0.0047 | - |
hexacarboxyporphyrinogen III | - |
Pseudomonas denitrificans (nom. rej.) | |
0.0063 | - |
S-adenosyl-L-methionine | - |
Pseudomonas denitrificans (nom. rej.) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | complete inhibition by 0.1 mM, mechanism unclear | Pseudomonas denitrificans (nom. rej.) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
SDS-PAGE | Pseudomonas denitrificans (nom. rej.) |
56000 | - |
gel filtration | Pseudomonas denitrificans (nom. rej.) |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + uroporphyrinogen III | Pseudomonas denitrificans (nom. rej.) | involved in biosynthesis of cobalamin | 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ | product is precorrin-2 | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas denitrificans (nom. rej.) | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0062 | - |
purified enzyme | Pseudomonas denitrificans (nom. rej.) |
Storage Stability | Organism |
---|---|
4°C, 10 mM Tris, pH 7.7, stable for several weeks | Pseudomonas denitrificans (nom. rej.) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + uroporphyrinogen III | - |
Pseudomonas denitrificans (nom. rej.) | 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ | produces equal amounts of precorrin-1 and precorrin-2 | ? | |
2 S-adenosyl-L-methionine + uroporphyrinogen III | - |
Pseudomonas denitrificans (nom. rej.) | 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ | product is precorrin-2 | ? | |
2 S-adenosyl-L-methionine + uroporphyrinogen III | involved in biosynthesis of cobalamin | Pseudomonas denitrificans (nom. rej.) | 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ | product is precorrin-2 | ? | |
additional information | uroporphyrin III is not a substrate of the enzyme | Pseudomonas denitrificans (nom. rej.) | ? | - |
? | |
S-adenosyl-L-methionine + heptacarboxyporphyrinogen III | - |
Pseudomonas denitrificans (nom. rej.) | ? | - |
? | |
S-adenosyl-L-methionine + hexacarboxyporphyrinogen III | - |
Pseudomonas denitrificans (nom. rej.) | ? | - |
? | |
S-adenosyl-L-methionine + pentacarboxyporphyrinogen III | - |
Pseudomonas denitrificans (nom. rej.) | ? | - |
? | |
S-adenosyl-L-methionine + uroporphyrinogen I | - |
Pseudomonas denitrificans (nom. rej.) | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | homodimer, 2 * 30000, SDS-PAGE, gel filtration | Pseudomonas denitrificans (nom. rej.) |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
38 | - |
uroporphyrinogen III | - |
Pseudomonas denitrificans (nom. rej.) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | 7.8 | - |
Pseudomonas denitrificans (nom. rej.) |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00032 | - |
S-adenosyl-L-homocysteine | - |
Pseudomonas denitrificans (nom. rej.) |