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Literature summary for 2.1.1.107 extracted from

  • Blanche, F.; Debussche, L.; Thibaut, D.; Crouzet, J.; Cameron, B.
    Purification and characterization of S-adenosyl-L-methionine: uroporphyrinogen III methyltransferase from Pseudomonas denitrificans (1989), J. Bacteriol., 171, 4222-4231.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
50 fold increase of enzyme activity when overexpressed Pseudomonas denitrificans (nom. rej.)

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by cobalamine or intermediates of synthesis Pseudomonas denitrificans (nom. rej.)
S-adenosyl-L-homocysteine competitive Pseudomonas denitrificans (nom. rej.)
uroporphyrinogen III substrate inhibition above 0.002 mM Pseudomonas denitrificans (nom. rej.)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
uroporphyrinogen III
-
Pseudomonas denitrificans (nom. rej.)
0.0034
-
pentacarboxyporphyrinogen III
-
Pseudomonas denitrificans (nom. rej.)
0.0042
-
heptacarboxyporphyrinogen III
-
Pseudomonas denitrificans (nom. rej.)
0.0047
-
hexacarboxyporphyrinogen III
-
Pseudomonas denitrificans (nom. rej.)
0.0063
-
S-adenosyl-L-methionine
-
Pseudomonas denitrificans (nom. rej.)

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ complete inhibition by 0.1 mM, mechanism unclear Pseudomonas denitrificans (nom. rej.)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
SDS-PAGE Pseudomonas denitrificans (nom. rej.)
56000
-
gel filtration Pseudomonas denitrificans (nom. rej.)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 S-adenosyl-L-methionine + uroporphyrinogen III Pseudomonas denitrificans (nom. rej.) involved in biosynthesis of cobalamin 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ product is precorrin-2 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas denitrificans (nom. rej.)
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0062
-
purified enzyme Pseudomonas denitrificans (nom. rej.)

Storage Stability

Storage Stability Organism
4°C, 10 mM Tris, pH 7.7, stable for several weeks Pseudomonas denitrificans (nom. rej.)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 S-adenosyl-L-methionine + uroporphyrinogen III
-
Pseudomonas denitrificans (nom. rej.) 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ produces equal amounts of precorrin-1 and precorrin-2 ?
2 S-adenosyl-L-methionine + uroporphyrinogen III
-
Pseudomonas denitrificans (nom. rej.) 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ product is precorrin-2 ?
2 S-adenosyl-L-methionine + uroporphyrinogen III involved in biosynthesis of cobalamin Pseudomonas denitrificans (nom. rej.) 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ product is precorrin-2 ?
additional information uroporphyrin III is not a substrate of the enzyme Pseudomonas denitrificans (nom. rej.) ?
-
?
S-adenosyl-L-methionine + heptacarboxyporphyrinogen III
-
Pseudomonas denitrificans (nom. rej.) ?
-
?
S-adenosyl-L-methionine + hexacarboxyporphyrinogen III
-
Pseudomonas denitrificans (nom. rej.) ?
-
?
S-adenosyl-L-methionine + pentacarboxyporphyrinogen III
-
Pseudomonas denitrificans (nom. rej.) ?
-
?
S-adenosyl-L-methionine + uroporphyrinogen I
-
Pseudomonas denitrificans (nom. rej.) ?
-
?

Subunits

Subunits Comment Organism
dimer homodimer, 2 * 30000, SDS-PAGE, gel filtration Pseudomonas denitrificans (nom. rej.)

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
38
-
uroporphyrinogen III
-
Pseudomonas denitrificans (nom. rej.)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7 7.8
-
Pseudomonas denitrificans (nom. rej.)

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00032
-
S-adenosyl-L-homocysteine
-
Pseudomonas denitrificans (nom. rej.)