Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta cells | Sorghum bicolor |
Crystallization (Comment) | Organism |
---|---|
in complex with S-adenosyl-L-methionine, hanging drop vapor diffusion method, using 0.2 M calcium acetate hydrate, 0.1 M Tris, pH 8.0, and 20% (w/v) PEG 4000 | Sorghum bicolor |
Protein Variants | Comment | Organism |
---|---|---|
D252A | inactive | Sorghum bicolor |
K180A | inactive | Sorghum bicolor |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | contains Ca2+ | Sorghum bicolor |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + caffeoyl-CoA | Sorghum bicolor | - |
S-adenosyl-L-homocysteine + feruloyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sorghum bicolor | C5Z4W3 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, Resource-Q column chromatography and hydroxyapatite column chromatography | Sorghum bicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with caffeic acid, 5-hydroxyferuloyl-CoA, feruloyl-CoA, 4-coumaroyl-CoA, and 5-hydroxyferuloyl-CoA | Sorghum bicolor | ? | - |
- |
|
S-adenosyl-L-methionine + caffeoyl-CoA | - |
Sorghum bicolor | S-adenosyl-L-homocysteine + feruloyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Sorghum bicolor |
Synonyms | Comment | Organism |
---|---|---|
CCoAOMT | - |
Sorghum bicolor |