Cloned (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
G46Y | mutation is sufficient for a reversal of the unusual para- back to meta-O-methylation of flavanones and dihydroflavonols | Arabidopsis thaliana |
G46Y/W193F/R194M/I195K | methylation in meta-position is preferred, mutations reduce the affinity for eriodictyol due to almost no interactions of the side chains with the substrate | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
eriodictyol | mutant G46Y, pH 7.5, 37°C | Arabidopsis thaliana | |
0.063 | - |
eriodictyol | wild-type, pH 7.5, 37°C | Arabidopsis thaliana | |
0.28 | - |
eriodictyol | mutant G46Y/W193F/R194M/I195K, pH 7.5, 37°C | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9C5D7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme shows a strong preference for methylating the para position of flavanones and dihydroflavonols, whereas flavones and flavonols are methylated in the meta-position | Arabidopsis thaliana | ? | - |
? | |
S-adenosyl-L-methionine + caffeic acid | 10% of the activity with luteolin | Arabidopsis thaliana | S-adenosyl-L-homocysteine + ferulic acid | synthesis of a single meta-methylated product | ? | |
S-adenosyl-L-methionine + caffeoyl-CoA | 33% of the activity with luteolin | Arabidopsis thaliana | S-adenosyl-L-homocysteine + feruloyl CoA | - |
? | |
S-adenosyl-L-methionine + dihydroquercetin | 2.5% of the activity with luteolin | Arabidopsis thaliana | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + eriodictyol | 15% of the activity with luteolin | Arabidopsis thaliana | S-adenosyl-L-homocysteine + eriodictoyl-3'-O-methylether + eriodictoyl-4'-O-methylether | 80% para product versus 20% meta in the wild type | ? | |
S-adenosyl-L-methionine + esculetin | 20% of the activity with luteolin | Arabidopsis thaliana | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + luteolin | - |
Arabidopsis thaliana | S-adenosyl-L-homocysteine + scoparol | synthesis of a single meta-methylated product | ? | |
S-adenosyl-L-methionine + quercetin | 60% of the activity with luteolin | Arabidopsis thaliana | S-adenosyl-L-homocysteine + isorhamnetin | synthesis of a single meta-methylated product | ? |
Synonyms | Comment | Organism |
---|---|---|
At4g26220 | - |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
eriodictyol | mutant G46Y, pH 7.5, 37°C | Arabidopsis thaliana | |
0.029 | - |
eriodictyol | mutant G46Y/W193F/R194M/I195K, pH 7.5, 37°C | Arabidopsis thaliana | |
0.08 | - |
eriodictyol | wild-type, pH 7.5, 37°C | Arabidopsis thaliana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.102 | - |
eriodictyol | mutant G46Y/W193F/R194M/I195K, pH 7.5, 37°C | Arabidopsis thaliana | |
0.92 | - |
eriodictyol | mutant G46Y, pH 7.5, 37°C | Arabidopsis thaliana | |
1.3 | - |
eriodictyol | wild-type, pH 7.5, 37°C | Arabidopsis thaliana |