Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli, pGEM-T easy vector, pCAMBIA1391xb vector, transgenic plants produced, promoter-GUS fusion constructs, T-DNA null mutant of GABI T-DNA insertion collection identified and characterized, double mutants lacking caffeoyl CoA 3-O-methyltransferase (CCoAOMT 1) and caffeic acid O-methyltransferase (COMT 1) | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + caffeoyl-CoA | Arabidopsis thaliana | enzyme predicted to catalyze first methylation step during biosynthesis of monolignols | S-adenosyl-L-homocysteine + feruloyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | O49499 | At4g34050; ecotypes Columbia (Col-0) and Wassilewskija (WS) | - |
Purification (Comment) | Organism |
---|---|
mutant and wild-type protein | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Arabidopsis thaliana | - |
stem | - |
Arabidopsis thaliana | - |
vascular tissue | interfascicular fibres | Arabidopsis thaliana | - |
xylem | - |
Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
T-DNA null mutant indicates collapsed xylem elements under short-day conditions, lignin composition of p-hydroxyphenyl units and inability of sinapoyl malate synthesis in double mutants impaired in caffeoyl CoA 3-O-methyltransferase (CCoAOMT 1) and caffeic acid O-methyltransferase (COMT 1), C3 methylation of the phenolic ring of monolignols in Arabidopsis mediated by both, caffeoyl CoA 3-O-methyltransferase (CCoAOMT 1) and caffeic acid O-methyltransferase (COMT 1), both enzymes also involved in formation of sinapoyl malate and isorhamnetin | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + caffeoyl-CoA | enzyme predicted to catalyze first methylation step during biosynthesis of monolignols | Arabidopsis thaliana | S-adenosyl-L-homocysteine + feruloyl-CoA | - |
? | |
S-adenosyl-L-methionine + caffeoyl-CoA | enzyme responsible for the first methylation step during biosynthesis of monolignols | Arabidopsis thaliana | S-adenosyl-L-homocysteine + feruloyl-CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CCoAOMT | - |
Arabidopsis thaliana |