Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.104 extracted from

  • Ferrer, J.L.; Zubieta, C.; Dixon, R.A.; Noel, J.P.
    Crystal structures of alfalfa caffeoyl coenzyme A 3-O-methyltransferase (2005), Plant Physiol., 137, 1009-1017.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
into the Escherichia coli expression vector pET-15b and transformed into Escherichia coli BL21(DE3) Medicago sativa

Crystallization (Commentary)

Crystallization (Comment) Organism
by vapor diffusion in hanging drops, at 2.45 A and 2.65 A resolution with CoA-linked substrates bound, and in complex with the reaction products Medicago sativa
vapor diffusion in hanging drops, enzyme in complex with the reaction products S-adenosine-L-homocysteine and feruloyl/sinapoyl CoAS Medicago sativa

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ in the active site of the enzyme surrounded by an octahedral arrangement of hydroxyl and carboxyl ligands, mediates the deprotonation of the caffeoyl 3-hydroxyl group and maintains the resultant oxoanion in close proximity to the reactive methyl group of S-adenosyl-L-methionine allowing for facile transmethylation to occur, restores activity of EDTA treated CCoAOMT to 100% the level of untreated CCoAOMT Medicago sativa
Mg2+ restores activity of EDTA treated CCoAOMT to 100% the level of untreated CCoAOMT Medicago sativa
Mn2+ only restores activity of EDTA treated CCoAOMT to 35% the level of untreated CCoAOMT Medicago sativa
Zn2+ restores activity of EDTA treated CCoAOMT to 100% the level of untreated CCoAOMT Medicago sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + caffeoyl-CoA Medicago sativa the enzyme is involved in lignin biosynthesis S-adenosyl-L-homocysteine + feruloyl-CoA
-
?

Organism

Organism UniProt Comment Textmining
Medicago sativa
-
-
-
Medicago sativa Q40313
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Medicago sativa
by sonication, centrifugation and Ni2+-NTA column, more than 98% pure Medicago sativa

Storage Stability

Storage Stability Organism
-80°C Medicago sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + caffeoyl-CoA
-
Medicago sativa S-adenosyl-L-homocysteine + feruloyl-CoA
-
?
S-adenosyl-L-methionine + caffeoyl-CoA the enzyme is involved in lignin biosynthesis Medicago sativa S-adenosyl-L-homocysteine + feruloyl-CoA
-
?

Subunits

Subunits Comment Organism
dimer crystallography Medicago sativa

Synonyms

Synonyms Comment Organism
caffeoyl coenzyme A 3-O-methyltransferase
-
Medicago sativa
CCoAOMT
-
Medicago sativa