Cloned (Comment) | Organism |
---|---|
into the Escherichia coli expression vector pET-15b and transformed into Escherichia coli BL21(DE3) | Medicago sativa |
Crystallization (Comment) | Organism |
---|---|
by vapor diffusion in hanging drops, at 2.45 A and 2.65 A resolution with CoA-linked substrates bound, and in complex with the reaction products | Medicago sativa |
vapor diffusion in hanging drops, enzyme in complex with the reaction products S-adenosine-L-homocysteine and feruloyl/sinapoyl CoAS | Medicago sativa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | in the active site of the enzyme surrounded by an octahedral arrangement of hydroxyl and carboxyl ligands, mediates the deprotonation of the caffeoyl 3-hydroxyl group and maintains the resultant oxoanion in close proximity to the reactive methyl group of S-adenosyl-L-methionine allowing for facile transmethylation to occur, restores activity of EDTA treated CCoAOMT to 100% the level of untreated CCoAOMT | Medicago sativa | |
Mg2+ | restores activity of EDTA treated CCoAOMT to 100% the level of untreated CCoAOMT | Medicago sativa | |
Mn2+ | only restores activity of EDTA treated CCoAOMT to 35% the level of untreated CCoAOMT | Medicago sativa | |
Zn2+ | restores activity of EDTA treated CCoAOMT to 100% the level of untreated CCoAOMT | Medicago sativa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + caffeoyl-CoA | Medicago sativa | the enzyme is involved in lignin biosynthesis | S-adenosyl-L-homocysteine + feruloyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Medicago sativa | - |
- |
- |
Medicago sativa | Q40313 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Medicago sativa |
by sonication, centrifugation and Ni2+-NTA column, more than 98% pure | Medicago sativa |
Storage Stability | Organism |
---|---|
-80°C | Medicago sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + caffeoyl-CoA | - |
Medicago sativa | S-adenosyl-L-homocysteine + feruloyl-CoA | - |
? | |
S-adenosyl-L-methionine + caffeoyl-CoA | the enzyme is involved in lignin biosynthesis | Medicago sativa | S-adenosyl-L-homocysteine + feruloyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | crystallography | Medicago sativa |
Synonyms | Comment | Organism |
---|---|---|
caffeoyl coenzyme A 3-O-methyltransferase | - |
Medicago sativa |
CCoAOMT | - |
Medicago sativa |