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Literature summary for 2.1.1.103 extracted from

  • Hirashima, T.; Toyoshima, M.; Moriyama, T.; Nakamura, Y.; Sato, N.
    Characterization of phosphoethanolamine-N-methyltransferases in green algae (2017), Biochem. Biophys. Res. Commun., 488, 141-146 .
    View publication on PubMed
Search for more literature for 2.1.1.103

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Top10 cells Chlamydomonas applanata
expressed in Escherichia coli Top10 cells Chlamydomonas asymmetrica
expression Escherichia coli Chlamydomonas applanata
expression Escherichia coli Chlamydomonas asymmetrica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0217
-
 Ethanolamine phosphate pH 8.0, 30°C Chlamydomonas applanata
0.0217
-
 Phosphoethanolamine at pH 8.0 and 30°C Chlamydomonas applanata
0.0292
-
 Ethanolamine phosphate pH 8.0, 30°C Chlamydomonas asymmetrica
0.0292
-
 Phosphoethanolamine at pH 8.0 and 30°C Chlamydomonas asymmetrica
0.0639
-
 S-adenosyl-L-methionine pH 8.0, 30°C Chlamydomonas applanata
0.0639
-
 S-adenosyl-L-methionine at pH 8.0 and 30°C Chlamydomonas applanata
0.106
-
 S-adenosyl-L-methionine pH 8.0, 30°C Chlamydomonas asymmetrica
0.106
-
 S-adenosyl-L-methionine at pH 8.0 and 30°C Chlamydomonas asymmetrica

Organism

Organism UniProt Comment Textmining
Chlamydomonas applanata A0A1W7HP20
-
-
Chlamydomonas applanata NIES-2202 A0A1W7HP20
-
-
Chlamydomonas asymmetrica A0A125SQH6
-
-
Chlamydomonas asymmetrica NIES-2207 A0A125SQH6
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography Chlamydomonas applanata
HisTrap column chromatography Chlamydomonas asymmetrica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no substrate: phosphomonomethylethanolamine Chlamydomonas applanata ?
-
-
additional information no substrate: phosphomonomethylethanolamine Chlamydomonas asymmetrica ?
-
-
additional information the enzyme shows no methyltransferase activity with phosphomonomethylethanolamine Chlamydomonas applanata ?
-
-
additional information the enzyme shows no methyltransferase activity with phosphomonomethylethanolamine Chlamydomonas asymmetrica ?
-
-
additional information no substrate: phosphomonomethylethanolamine Chlamydomonas asymmetrica NIES-2207 ?
-
-
additional information the enzyme shows no methyltransferase activity with phosphomonomethylethanolamine Chlamydomonas asymmetrica NIES-2207 ?
-
-
additional information no substrate: phosphomonomethylethanolamine Chlamydomonas applanata NIES-2202 ?
-
-
additional information the enzyme shows no methyltransferase activity with phosphomonomethylethanolamine Chlamydomonas applanata NIES-2202 ?
-
-
S-adenosyl-L-methionine + ethanolamine phosphate
-
 Chlamydomonas applanata S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
-
 ?
S-adenosyl-L-methionine + ethanolamine phosphate
-
 Chlamydomonas asymmetrica S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
-
 ?
S-adenosyl-L-methionine + ethanolamine phosphate
-
 Chlamydomonas asymmetrica NIES-2207 S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
-
 ?
S-adenosyl-L-methionine + ethanolamine phosphate
-
 Chlamydomonas applanata NIES-2202 S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
-
 ?
S-adenosyl-L-methionine + phosphoethanolamine
-
 Chlamydomonas applanata S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
-
 ?
S-adenosyl-L-methionine + phosphoethanolamine
-
 Chlamydomonas asymmetrica S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
-
 ?
S-adenosyl-L-methionine + phosphoethanolamine
-
 Chlamydomonas asymmetrica NIES-2207 S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
-
 ?
S-adenosyl-L-methionine + phosphoethanolamine
-
 Chlamydomonas applanata NIES-2202 S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 67900, calculated from amino acid sequence Chlamydomonas applanata
? x * 69700, SDS-PAGE, thioredoxin-tagged recombinant protein Chlamydomonas applanata
? x * 75300, calculated from amino acid sequence Chlamydomonas asymmetrica
? x * 75300, SDS-PAGE, thioredoxin-tagged recombinant protein Chlamydomonas asymmetrica

Synonyms

Synonyms Comment Organism
PEAMT
-
 Chlamydomonas applanata
PEAMT
-
 Chlamydomonas asymmetrica
phosphoethanolamine-N-methyltransferases
-
 Chlamydomonas applanata
phosphoethanolamine-N-methyltransferases
-
 Chlamydomonas asymmetrica