Literature summary for 2.1.1.103 extracted from

Garg, A.; Lukk, T.; Kumar, V.; Choi, J.Y.; Augagneur, Y.; Voelker, D.R.; Nair, S.; Ben Mamoun, C.
Structure, function and inhibition of the phosphoethanolamine methyltransferases of the human malaria parasites Plasmodium vivax and Plasmodium knowlesi (2015), Sci. Rep., 5, 9064.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Plasmodium knowlesi
expressed in Escherichia coli BL21(DE3) cells	Plasmodium vivax

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 100 mM Na-acetate (pH 5.0), and 20% (w/v) PEG 3350	Plasmodium knowlesi
sitting drop vapor diffusion method, using 200 mM NH4-formate and 20% (w/v) PEG 3350	Plasmodium vivax

Inhibitors

Inhibitors	Comment	Organism	Structure
amodiaquine	-	Plasmodium knowlesi
amodiaquine	-	Plasmodium vivax
Chloroquine	-	Plasmodium knowlesi
Chloroquine	-	Plasmodium vivax
NSC158011	-	Plasmodium knowlesi
NSC158011	-	Plasmodium vivax

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.1	-	Ethanolamine phosphate	at pH 8.6 and 37°C	Plasmodium knowlesi
0.21	-	Ethanolamine phosphate	at pH 8.6 and 37°C	Plasmodium vivax
0.23	-	S-adenosyl-L-methionine	at pH 8.6 and 37°C	Plasmodium vivax
0.27	-	S-adenosyl-L-methionine	at pH 8.6 and 37°C	Plasmodium knowlesi

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
31000	-	x * 31000, SDS-PAGE	Plasmodium knowlesi
31000	-	x * 31000, SDS-PAGE	Plasmodium vivax

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + ethanolamine phosphate	Plasmodium knowlesi	-	S-adenosyl-L-homocysteine + N-methylethanolamine phosphate	-	?
S-adenosyl-L-methionine + ethanolamine phosphate	Plasmodium vivax	-	S-adenosyl-L-homocysteine + N-methylethanolamine phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Plasmodium knowlesi	B3L8G9	-	-
Plasmodium vivax	A5K867	-	-

Purification (Commentary)

Purification (Comment)	Organism
nickel HisTrap column chromatography and Superdex 75PG gel filtration	Plasmodium knowlesi
nickel HisTrap column chromatography and Superdex 75PG gel filtration	Plasmodium vivax

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + ethanolamine phosphate	-	Plasmodium knowlesi	S-adenosyl-L-homocysteine + N-methylethanolamine phosphate	-	?
S-adenosyl-L-methionine + ethanolamine phosphate	-	Plasmodium vivax	S-adenosyl-L-homocysteine + N-methylethanolamine phosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 31000, SDS-PAGE	Plasmodium knowlesi
?	x * 31000, SDS-PAGE	Plasmodium vivax

Synonyms

Synonyms	Comment	Organism
phosphoethanolamine methyltransferase	-	Plasmodium knowlesi
phosphoethanolamine methyltransferase	-	Plasmodium vivax
PMT	-	Plasmodium knowlesi
PMT	-	Plasmodium vivax

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.05	-	at pH 8.6 and 37°C	Plasmodium knowlesi	NSC158011
0.099	-	at pH 8.6 and 37°C	Plasmodium vivax	NSC158011
0.146	-	at pH 8.6 and 37°C	Plasmodium knowlesi	Chloroquine
0.146	-	at pH 8.6 and 37°C	Plasmodium vivax	Chloroquine
0.22	-	at pH 8.6 and 37°C	Plasmodium vivax	amodiaquine
0.398	-	at pH 8.6 and 37°C	Plasmodium knowlesi	amodiaquine

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Release 2023.1 (January 2023)