Literature summary for 2.1.1.103 extracted from

Lee, S.G.; Kim, Y.; Alpert, T.D.; Nagata, A.; Jez, J.M.
Structure and reaction mechanism of phosphoethanolamine methyltransferase from the malaria parasite Plasmodium falciparum: an antiparasitic drug target (2012), J. Biol. Chem., 287, 1426-1434.

Search for more literature for 2.1.1.103

Application

Application	Comment	Organism
medicine	the enzyme is an antiparasitic target	Plasmodium falciparum

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Plasmodium falciparum

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with either S-adenosyl-L-methionine (1), phosphoethanolamine (2), phosphocholine (3), sinefungin (4), or both phosphoethanolamine and S-adenosylhomocysteine (5), hanging drop vapor diffusion method, using (1) 20% (w/v) PEG-8000, 0.1 M sodium cacodylate, pH 6.5, 0.2 M sodium acetate, 20 mM tris(2-carboxyethyl) phosphine, or (2-5) 20-30% (w/v) PEG8000, 0.1 M sodium cacodylate,pH 6.5, 0.2 M sodium acetate, 5 mM 2-mercaptoethanol	Plasmodium falciparum

Crystallization (Comment)

Organism

in complex with either S-adenosyl-L-methionine (1), phosphoethanolamine (2), phosphocholine (3), sinefungin (4), or both phosphoethanolamine and S-adenosylhomocysteine (5), hanging drop vapor diffusion method, using (1) 20% (w/v) PEG-8000, 0.1 M sodium cacodylate, pH 6.5, 0.2 M sodium acetate, 20 mM tris(2-carboxyethyl) phosphine, or (2-5) 20-30% (w/v) PEG8000, 0.1 M sodium cacodylate,pH 6.5, 0.2 M sodium acetate, 5 mM 2-mercaptoethanol

Plasmodium falciparum

Protein Variants

Protein Variants	Comment	Organism
D110A	the mutant shows about 37% of wild type activity	Plasmodium falciparum
D110N	the mutant shows about 80% of wild type activity	Plasmodium falciparum
D61A	the mutant shows about 35% of wild type activity	Plasmodium falciparum
D61N	the mutant shows about 68% of wild type activity	Plasmodium falciparum
D85A	inactive	Plasmodium falciparum
D85N	inactive	Plasmodium falciparum
H132A	inactive	Plasmodium falciparum
H132N	inactive	Plasmodium falciparum
H132Q	inactive	Plasmodium falciparum
I86A	the mutant shows about 10% of wild type activity	Plasmodium falciparum
I86F	the mutant shows about 16% of wild type activity	Plasmodium falciparum
K247A	inactive	Plasmodium falciparum
K247M	inactive	Plasmodium falciparum
Q18N	the mutant shows about 20% of wild type activity	Plasmodium falciparum
R179A	inactive	Plasmodium falciparum
R179A	the mutant shows about 5% of wild type activity	Plasmodium falciparum
R179K	the mutant shows about 45% of wild type activity	Plasmodium falciparum
S37A	the mutant shows about 85% of wild type activity	Plasmodium falciparum
Y160A	inactive	Plasmodium falciparum
Y160F	inactive	Plasmodium falciparum
Y175A	the mutant shows about 10% of wild type activity	Plasmodium falciparum
Y175F	the mutant shows about 9% of wild type activity	Plasmodium falciparum
Y181A	the mutant shows about 7% of wild type activity	Plasmodium falciparum
Y181F	the mutant shows about 5% of wild type activity	Plasmodium falciparum
Y19A	inactive	Plasmodium falciparum
Y19F	the mutant shows about 30% of wild type activity	Plasmodium falciparum
Y27A	the mutant shows about 5% of wild type activity	Plasmodium falciparum
Y27F	the mutant shows about 8% of wild type activity	Plasmodium falciparum

Inhibitors

Inhibitors	Comment	Organism	Structure
sinefungin	-	Plasmodium falciparum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.0299	-	S-adenosyl-L-methionine	using phosphoethanolamine as cosubstrate pH and temperature not specified in the publication	Plasmodium falciparum
0.0331	-	S-adenosyl-L-methionine	using phosphodimethylethanolamine as cosubstrate, pH and temperature not specified in the publication	Plasmodium falciparum
0.0542	-	Phosphoethanolamine	pH and temperature not specified in the publication	Plasmodium falciparum
0.0668	-	phosphodimethylethanolamine	pH and temperature not specified in the publication	Plasmodium falciparum
0.181	-	phosphomonomethylethanolamine	pH and temperature not specified in the publication	Plasmodium falciparum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Organism

Organism	UniProt	Comment	Textmining
Plasmodium falciparum	Q6T755	-	-

Purification (Commentary)

Purification (Comment)	Organism
nickel-affinity column chromatography and gel filtration	Plasmodium falciparum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
3 S-adenosyl-L-methionine + phosphoethanolamine	overall reaction	Plasmodium falciparum	3 S-adenosyl-L-homocysteine + phosphocholine	-	?
S-adenosyl-L-methionine + phosphodimethylethanolamine	-	Plasmodium falciparum	S-adenosyl-L-homocysteine + phosphocholine	-	?
S-adenosyl-L-methionine + phosphoethanolamine	-	Plasmodium falciparum	S-adenosyl-L-homocysteine + phosphomonomethylethanolamine	-	?
S-adenosyl-L-methionine + phosphomonomethylethanolamine	-	Plasmodium falciparum	S-adenosyl-L-homocysteine + phosphodimethylethanolamine	-	?

Synonyms

Synonyms	Comment	Organism
phosphoethanolamine methyltransferase	-	Plasmodium falciparum
PMT	-	Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
0.39	-	S-adenosyl-L-methionine	using phosphoethanolamine as cosubstrate pH and temperature not specified in the publication	Plasmodium falciparum
0.49	-	S-adenosyl-L-methionine	using phosphodimethylethanolamine as cosubstrate, pH and temperature not specified in the publication	Plasmodium falciparum
1.82	-	Phosphoethanolamine	pH and temperature not specified in the publication	Plasmodium falciparum
3.08	-	phosphodimethylethanolamine	pH and temperature not specified in the publication	Plasmodium falciparum
3.63	-	phosphomonomethylethanolamine	pH and temperature not specified in the publication	Plasmodium falciparum

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism
13.1	-	S-adenosyl-L-methionine	using phosphoethanolamine as cosubstrate pH and temperature not specified in the publication	Plasmodium falciparum
14.7	-	S-adenosyl-L-methionine	using phosphodimethylethanolamine as cosubstrate, pH and temperature not specified in the publication	Plasmodium falciparum
20.07	-	phosphomonomethylethanolamine	pH and temperature not specified in the publication	Plasmodium falciparum
33.52	-	Phosphoethanolamine	pH and temperature not specified in the publication	Plasmodium falciparum
46.16	-	phosphodimethylethanolamine	pH and temperature not specified in the publication	Plasmodium falciparum