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Literature summary for 2.1.1.100 extracted from

  • Yang, J.; Kulkarni, K.; Manolaridis, I.; Zhang, Z.; Dodd, R.B.; Mas-Droux, C.; Barford, D.
    Mechanism of isoprenylcysteine carboxyl methylation from the crystal structure of the integral membrane methyltransferase ICMT (2011), Mol. Cell, 44, 997-1004.
    View publication on PubMed
Search for more literature for 2.1.1.100

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli C41(DE3) cells Methanosarcina acetivorans

Protein Variants

Protein Variants Comment Organism
E167A the mutation leads to strongly reduced activity compared to the wild type enzyme Methanosarcina acetivorans
H113A the mutation leads to strongly reduced activity compared to the wild type enzyme Methanosarcina acetivorans
H126A the mutation leads to strongly reduced activity compared to the wild type enzyme Methanosarcina acetivorans
R163A the mutation leads to strongly reduced activity compared to the wild type enzyme Methanosarcina acetivorans

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane integral membrane protein Methanosarcina acetivorans 16020
-

Organism

Organism UniProt Comment Textmining
Methanosarcina acetivorans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, ion exchange column chromatography, and gel filtration Methanosarcina acetivorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + N-acetyl-S-[(2E,6E)-farnesyl]-L-cysteine
-
 Methanosarcina acetivorans S-adenosyl-L-homocysteine + N-acetyl-S-[(2E,6E)-farnesyl]-L-cysteine methyl ester
-
 ?
S-adenosyl-L-methionine + S-[(2E,6E)-farnesyl]-sulfanylacetic acid
-
 Methanosarcina acetivorans S-adenosyl-L-homocysteine + methyl S-[(2E,6E)-farnesyl]-sulfanylacetate
-
 ?

Synonyms

Synonyms Comment Organism
Icmt
-
 Methanosarcina acetivorans
isoprenylcysteine carboxyl methyltransferase
-
 Methanosarcina acetivorans