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Literature summary for 2.1.1.100 extracted from

  • Wright, L.P.; Court, H.; Mor, A.; Ahearn, I.M.; Casey, P.J.; Philips, M.R.
    Topology of mammalian isoprenylcysteine carboxyl methyltransferase determined in live cells with a fluorescent probe (2009), Mol. Cell. Biol., 29, 1826-1833.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
Icmt, expression in HEK-293 cells and Cos-1 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
D122A site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme, comparison of membrane topology Homo sapiens
E107A site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme, comparison of membrane topology Homo sapiens
E142A site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme, comparison of membrane topology Homo sapiens
E146A site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme, comparison of membrane topology Homo sapiens
E251A site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme, comparison of membrane topology Homo sapiens
E252A site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme, comparison of membrane topology Homo sapiens
F124A site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme, comparison of membrane topology Homo sapiens
F124R site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme, comparison of membrane topology Homo sapiens
F258A site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme, comparison of membrane topology Homo sapiens
F72A site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme, comparison of membrane topology Homo sapiens
additional information construction of various CFP-Icmt C-terminal truncation mutants, overview, comparison of membrane topology Homo sapiens
P275A site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme, comparison of membrane topology Homo sapiens
R209A site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme, comparison of membrane topology Homo sapiens
R63A site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme, comparison of membrane topology Homo sapiens
RH209/210A site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme, comparison of membrane topology Homo sapiens
S123A site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme, comparison of membrane topology Homo sapiens
W241A site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme, comparison of membrane topology Homo sapiens
Y266A site-directed mutagenesis, the mutant shows increased activity comapred to the wild-type enzyme, comparison of membrane topology Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum membrane integral membrane protein with 6 transmembrane segments, determination and analysis of membrane topology using recombinant Icmt expressed in COS-1 cells, overview Homo sapiens 5789
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Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
activities of wild-type and mutant enzymes expressed in HEK-293 cells Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + N-biotinyl-S-farnesyl-L-cysteine peptide
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Homo sapiens S-adenosyl-L-homocysteine + biotin S-farnesyl-L-cysteine peptide methyl ester
-
?

Synonyms

Synonyms Comment Organism
Icmt
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Homo sapiens
isoprenylcysteine carboxyl methyltransferase
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Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at room temperature Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
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Homo sapiens