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Literature summary for 2.1.1.10 extracted from

  • Neuhierl, B.; Thanbichler, M.; Lottspeich, F.; B÷ck, A.
    A family of S-methylmethionine-dependent thiol/selenol methyltransferases. Role in selenium tolerance and evolutionary relation (1999), J. Biol. Chem., 274, 5407-5414.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
L-methionine product inhibition above 0.5 mM Escherichia coli
S-adenosyl-L-methionine product inhibition above 0.25 mM Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.045
-
L-homocysteine pH 6, 30┬░C, cosubstrate: S-adenosyl-L-methionine Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32500
-
1 * 32500, calculated from sequence Escherichia coli
34000
-
gel filtration Escherichia coli
35000
-
1 * 35000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-methyl-L-methionine + L-homocysteine Escherichia coli
-
2 L-methionine
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli Q47690
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme also catalyzes the reaction of selenocysteine methyltransferase Escherichia coli ?
-
?
S-adenosyl-L-methionine + L-homocysteine
-
Escherichia coli S-adenosyl-L-homocysteine + L-methionine
-
?
S-methyl-L-methionine + L-homocysteine
-
Escherichia coli 2 L-methionine
-
?

Subunits

Subunits Comment Organism
monomer 1 * 35000, SDS-PAGE Escherichia coli
monomer 1 * 32500, calculated from sequence Escherichia coli

Synonyms

Synonyms Comment Organism
YagD
-
Escherichia coli
YagD protein
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.38
-
L-homocysteine pH 6, 30┬░C, cosubstrate: S-adenosyl-L-methionine Escherichia coli