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Literature summary for 2.1.1.1 extracted from

  • van Haren, M.J.; Thomas, M.G.; Sartini, D.; Barlow, D.J.; Ramsden, D.B.; Emanuelli, M.; Klamt, F.; Martin, N.I.; Parsons, R.B.
    The kinetic analysis of the N-methylation of 4-phenylpyridine by nicotinamide N-methyltransferase Evidence for a novel mechanism of substrate inhibition (2018), Int. J. Biochem. Cell Biol., 98, 127-136 .
    View publication on PubMed
Search for more literature for 2.1.1.1

Crystallization (Commentary)

Crystallization (Comment) Organism
in silico molecular docking. Substrate 4-phenylpyridine binds to the active site of NNMT in two non-redundant poses, one a substrate binding mode and the other an inhibitory mode Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Dimethylsulfoxide competitive, about 82% inhibition at 1% v/v Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.125
-
 4-phenylpyridine pH not specified in the publication, temperature not specified in the publication Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P40261
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000028
-
 substrate 1-methyl-4-phenylpyridine, pH not specified in the publication, temperature not specified in the publication Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 4-phenylpyridine poor substrate Homo sapiens S-adenosyl-L-homocysteine + 1-methyl-4-phenylpyridine
-
 ?

Synonyms

Synonyms Comment Organism
NNMT
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000093
-
 4-phenylpyridine pH not specified in the publication, temperature not specified in the publication Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4
-
 Dimethylsulfoxide substrate concentration above 2.5 mM, pH not specified in the publication, temperature not specified in the publication Homo sapiens
34
-
 Dimethylsulfoxide substrate concentration below 2.5 mM, pH not specified in the publication, temperature not specified in the publication Homo sapiens

General Information

General Information Comment Organism
physiological function the expression of NNMT in the SH-SY5Y cell-line has no effect on cell death, viability, ATP content or mitochondrial membrane potential Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0008
-
 4-phenylpyridine pH not specified in the publication, temperature not specified in the publication Homo sapiens