Crystallization (Comment) | Organism |
---|---|
in silico molecular docking. Substrate 4-phenylpyridine binds to the active site of NNMT in two non-redundant poses, one a substrate binding mode and the other an inhibitory mode | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Dimethylsulfoxide | competitive, about 82% inhibition at 1% v/v | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.125 | - |
4-phenylpyridine | pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P40261 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000028 | - |
substrate 1-methyl-4-phenylpyridine, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 4-phenylpyridine | poor substrate | Homo sapiens | S-adenosyl-L-homocysteine + 1-methyl-4-phenylpyridine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NNMT | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000093 | - |
4-phenylpyridine | pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
4 | - |
Dimethylsulfoxide | substrate concentration above 2.5 mM, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
34 | - |
Dimethylsulfoxide | substrate concentration below 2.5 mM, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | the expression of NNMT in the SH-SY5Y cell-line has no effect on cell death, viability, ATP content or mitochondrial membrane potential | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0008 | - |
4-phenylpyridine | pH not specified in the publication, temperature not specified in the publication | Homo sapiens |