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Literature summary for 2.1.1.1 extracted from

  • Peng, Y.; Sartini, D.; Pozzi, V.; Wilk, D.; Emanuelli, M.; Yee, V.C.
    Structural basis of substrate recognition in human nicotinamide N-methyltransferase (2011), Biochemistry, 50, 7800-7808.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
His-tagged K100A/E101A/E103A mutant, sitting drop vapor diffusion method, PEG3350 Homo sapiens

Protein Variants

Protein Variants Comment Organism
D197A active site residue Homo sapiens
K100A/E101A/E103A His-tagged version, crystallization facilitated by reducing the entropy of three surface exposed residues Homo sapiens
S201A active site residue Homo sapiens
S213A active site residue Homo sapiens
Y20A active site residue Homo sapiens
Y20F active site residue Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
S-adenosyl-L-methionine 0.0018-0.0022 mM, wild type protein, pH 8.6, 37°C Homo sapiens
0.005
-
S-adenosyl-L-methionine K100A/E101A/E103A mutant protein, pH 8.6, 37°C Homo sapiens
0.028
-
S-adenosyl-L-methionine Y20F mutant protein, pH 8.6, 37°C Homo sapiens
0.105
-
nicotinamide K100A/E101A/E103A mutant protein, pH 8.6, 37°C Homo sapiens
0.24
-
nicotinamide Y20F mutant protein, pH 8.6, 37°C Homo sapiens
0.4
-
nicotinamide 0.43-0.38 mM, wild type protein, pH 8.6, 37°C Homo sapiens
0.63
-
nicotinamide Y20A mutant protein, pH 8.6, 37°C Homo sapiens
2.73
-
S-adenosyl-L-methionine D197A mutant protein, pH 8.6, 37°C Homo sapiens
3.5
-
S-adenosyl-L-methionine Y20A mutant protein, pH 8.6, 37°C Homo sapiens
11.8
-
nicotinamide D197A mutant protein, pH 8.6, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P40261
-
-

Purification (Commentary)

Purification (Comment) Organism
His-tagged K100A/E101A/E103A mutant, immobilized metal ion affinity chromatography (Ni2+), gel filtration Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0011
-
Y20A mutant protein, pH 8.6, 37°C Homo sapiens
0.0042
-
D197A mutant protein, pH 8.6, 37°C Homo sapiens
0.077
-
Y20F mutant protein, pH 8.6, 37°C Homo sapiens
0.14
-
S201A mutant protein, pH 8.6, 37°C Homo sapiens
0.15
-
wild type protein, pH 8.6, 37°C Homo sapiens
0.18
-
K100A/E101A/E103A mutant protein, pH 8.6, 37°C Homo sapiens
0.21
-
S213A mutant protein, pH 8.6, 37°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nicotinamide + S-adenosyl-L-methionine
-
Homo sapiens 1-methylnicotinamide + S-adenosyl-L-homocysteine
-
?

Synonyms

Synonyms Comment Organism
nicotinamide N-methyltransferase
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.031
-
nicotinamide Y20A mutant protein, pH 8.6, 37°C Homo sapiens
0.036
-
nicotinamide D197A mutant protein, pH 8.6, 37°C Homo sapiens
0.041
-
nicotinamide Y20F mutant protein, pH 8.6, 37°C Homo sapiens
0.077
-
nicotinamide 0.43-0.38 mM, wild type protein, pH 8.6, 37°C Homo sapiens
0.083
-
nicotinamide K100A/E101A/E103A mutant protein, pH 8.6, 37°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.003
-
nicotinamide D197A mutant protein, pH 8.6, 37°C Homo sapiens
0.005
-
nicotinamide Y20A mutant protein, pH 8.6, 37°C Homo sapiens
0.009
-
S-adenosyl-L-methionine Y20A mutant protein, pH 8.6, 37°C Homo sapiens
0.013
-
S-adenosyl-L-methionine D197A mutant protein, pH 8.6, 37°C Homo sapiens
0.17
-
nicotinamide Y20F mutant protein, pH 8.6, 37°C Homo sapiens
0.19
-
nicotinamide 0.18-0.2 1/sec*mM, wild type protein, pH 8.6, 37°C Homo sapiens
0.8
-
nicotinamide K100A/E101A/E103A mutant protein, pH 8.6, 37°C Homo sapiens
1.5
-
S-adenosyl-L-methionine Y20F mutant protein, pH 8.6, 37°C Homo sapiens
17
-
S-adenosyl-L-methionine K100A/E101A/E103A mutant protein, pH 8.6, 37°C Homo sapiens
39
-
S-adenosyl-L-methionine 35-43 1/sec*mM, wild type protein, pH 8.6, 37°C Homo sapiens