Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.97.1.9 extracted from

  • Yee, N.; Choi, J.; Porter, A.W.; Carey, S.; Rauschenbach, I.; Harel, A.
    Selenate reductase activity in Escherichia coli requires Isc iron-sulfur cluster biosynthesis genes (2014), FEMS Microbiol. Lett., 361, 138-143 .
    View publication on PubMed
Search for more literature for 1.97.1.9

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli reduction of Se(VI) to Se(0) via Se(IV) ?
-
 ?
selenate + reduced acceptor Escherichia coli
-
 selenite + H2O + acceptor
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 ECISC-202
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information reduction of Se(VI) to Se(0) via Se(IV) Escherichia coli ?
-
 ?
selenate + reduced acceptor
-
 Escherichia coli selenite + H2O + acceptor
-
 ?

Subunits

Subunits Comment Organism
More the selenate reductase in Escherichia coli is a multisubunit enzyme predicted to bind Fe-S clusters Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 in vivo assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
iron-sulfur center activity of genes iscU or hscB in the Isc iron-sulfur cluster biosynthesis pathway is required for formation of selenate reductase activity in vivo Escherichia coli

General Information

General Information Comment Organism
metabolism activity of genes iscU or hscB in the Isc iron-sulfur cluster biosynthesis pathway is required for formation of selenate reductase activity in vivo. Mutant strains JW2513 and JW2511 devoid of either the iscU or hscB gene in the Isc iron–sulfur cluster biosynthesis pathway lose the ability to reduce selenate, phenotypes, overview. Genetic complementation by the wildtype sequences restored selenate reductase activity. The Isc biosynthetic system plays a key role in selenate reductase Fe-S cofactor assembly and is essential for enzyme activity Escherichia coli